Regional differences in mitral cell development in mouse olfactory bulb.
Regional differences in mitral cell development in mouse olfactory bulb.
复制标题
小鼠嗅球二尖瓣细胞发育的区域差异。
DOI：
10.1002/cne.24683
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Imamura,Fumiaki
中科院分区：
文献类型：
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作者：
Nguyen,UyenP;Imamura,Fumiaki
位于嗅上皮背内侧和腹内侧区域的嗅感觉神经元（OSN）基于其分子表达模式而彼此区分。这种差异反映在分离成背内侧和腹外侧区域的嗅球（OB）的肾小球层。然而，目前还不清楚是否互补的分离也很明显，在投射神经元支配的OB肾小球。在这项研究中，我们比较了不同地区之间的OB的发展，通过专注于转录因子，Tbx 21，这是由二尖瓣和簇状细胞在成熟的OB表达。检查不同发育年龄的OB，我们发现Tbx 21表达开始于称为舌形区域的前内侧区域，然后是背内侧区域，然后是腹外侧区域。我们还发现，舌形区由位于腹外侧OE（V区：DM）最背内侧部分的OSN支配。有趣的是，OSN的产生首先发生在背内侧区，包括V区：DM，表明OE中OSN产生的时间过程与OB靶区域中Tbx 21表达之间存在相关性。相比之下，vGluT 1的表达，这也是在所有的二尖瓣细胞中发现的成熟OB，首先检测到在腹外侧区在发展过程中。我们的研究结果表明，投射神经元的发育以区室化的方式发生在OB;舌形，背内侧和腹外侧区域，并且并非所有投射神经元都遵循相同的发育途径。
Olfactory sensory neurons (OSNs) located in the dorsomedial and ventromedial regions of the olfactory epithelium (OE) are distinguished from one another based on their molecular expression patterns. This difference is reflected in the separation of the glomerular layer of the olfactory bulb (OB) into dorsomedial and ventrolateral regions. However, it is unclear whether a complementary separation is also evident in the projection neurons that innervate the OB glomeruli. In this study, we compared the development of the OB between different regions by focusing on the transcription factor, Tbx21, which is expressed by mitral and tufted cells in the mature OB. Examining the OB at different developmental ages, we found that Tbx21 expression commenced in the anteromedial region called the tongue‐shaped area, followed by the dorsomedial and then ventrolateral areas. We also showed that the tongue‐shaped area was innervated by the OSNs located in the most dorsomedial part of the ventrolateral OE, the V‐zone:DM. Interestingly, the generation of OSNs occurred first in the dorsomedial zone including the V‐zone:DM, suggesting a correlation between the time course of OSN generation in the OE and Tbx21 expression in their target region of the OB. In contrast, expression of vGluT1, which is also found in all mitral cells in the mature OB, was first detected in the ventrolateral region during development. Our findings demonstrate that the development of projection neurons occurs in a compartmentalized manner in the OB; tongue‐shaped, dorsomedial, and ventrolateral areas, and that not all projection neurons follow the same developmental pathway.