LEUKOCYTE CHEMOTAXIS UNDER AGAROSE - MANIPULATIONS OF SERUM AND PLASMA BEFORE INCORPORATION INTO AGAROSE CAN INFLUENCE CELL-MOVEMENT
LEUKOCYTE CHEMOTAXIS UNDER AGAROSE - MANIPULATIONS OF SERUM AND PLASMA BEFORE INCORPORATION INTO AGAROSE CAN INFLUENCE CELL-MOVEMENT
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DOI：
10.1016/0022-1759(79)90001-2
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发表时间：
1979-01-01
影响因子：
2.2
通讯作者：
LINDROOS, WE
中科院分区：
文献类型：
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作者：
DAHL, MV;LINDROOS, WE
在用于测量多形核白细胞趋化性和随机迁移的琼脂糖迁移测定中，琼脂糖凝胶通常含有10％的人血清或血浆。使用该测定确定了血清和血浆对细胞运动的影响。与使用血清相比，利用血浆作为蛋白质来源可以实现更多的随机迁移和趋化性。血清和血浆的热灭活降低了随机迁移和趋化性。如果加热到56°C。 C延长超过15-30分钟，白细胞运动增强。琼脂糖中血小板、血小板产品或肝素浓度过高也会增强迁移，但血浆或血清在不同温度下储存 24 小时不会明显影响迁移。琼脂糖中大量的 Hb 会降低趋化性，但微量溶血的影响可以忽略不计。在用于琼脂糖下测定之前以标准方式处理血浆或血清可以使该测定更具重现性和敏感性。
In the migration under agarose assay for measurement of polymorphonuclear leukocyte chemotaxis and random migration, the agarose gel ordinarily contains 10% human serum or plasma. Influences of serum and plasma on cell movement using this assay were defined. Utilizing plasma as protein source allowed more random migration and chemotaxis than when serum was used. Heat inactivation of serum and plasma decreased random migration and chemotaxis. If heating at 56.degree. C was prolonged beyond 15-30 min, leukocyte movement was augmented. Excessive concentrations of platelets, platelet products or heparin in agarose also augmented migration, but storage of plasma or sera at various temperatures for 24 h did not appreciably affect migration. Large amounts of Hb in agarose decreased chemotaxis, but the effect of trace amounts of hemolysis was negligible. Handling plasma or sera in standard ways prior to use in the under agarose assay may make this assay more reproducible and sensitive.