The FpPPR1 Gene Encodes a Pentatricopeptide Repeat Protein That Is Essential for Asexual Development, Sporulation, and Pathogenesis in Fusarium pseudograminearum.
The FpPPR1 Gene Encodes a Pentatricopeptide Repeat Protein That Is Essential for Asexual Development, Sporulation, and Pathogenesis in Fusarium pseudograminearum.
复制标题
FpPPR1 基因编码五肽重复蛋白，该蛋白对于镰孢菌的无性发育、孢子形成和发病机制至关重要
DOI：
10.3389/fgene.2020.535622
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发表时间：
2020
影响因子：
3.7
通讯作者：
Li H
中科院分区：
文献类型：
--
作者：
Wang L;Xie S;Zhang Y;Kang R;Zhang M;Wang M;Li H;Chen L;Yuan H;Ding S;Liang S;Li H
镰刀菌冠腐病（FCR）和赤霉病（FHB）是由假禾谷镰刀菌（Fusarium pseudograminearum）引起的小麦新病害。在这项研究中，我们的特点FpPPR 1，一个基因，编码一种蛋白质与12个五肽重复（PPR）基序。Δ Fpppr 1缺失突变株在马铃薯葡萄糖琼脂平板上的径向生长速率显著低于野生型菌株WZ-8A，并且具有扇形突变的菌落显著较小。突变体的气生菌丝在培养管中几乎不存在。Δ Fpppr 1突变体能够产生孢子，但产生的孢子大小异常，分生孢子隔膜形状改变，与野生型相比孢子形成显著减少。用菌丝栓或孢子悬液接种小麦胚芽鞘和大麦叶片，Δ Fpppr 1均未致病。在补充菌株中，突变表型成功恢复到野生型水平。FpPpr 1-GFP在孢子和菌丝中的信号主要与Mito-tracker信号重叠，这证实了FpPpr 1的线粒体靶向信号预测。RNAseq显示Δ Fpppr 1突变体中有1，367个基因下调，1，333个基因上调。NAD结合蛋白、硫氧还蛋白、2Fe-2S铁硫簇结合结构域蛋白和细胞色素P450基因在Δ Fpppr 1中显著下调，表明突变体中的谷氨酸介导的还原酶氧化还原应激功能障碍。交配型自形等位基因MAT 1 -1-1、MAT 1 -1-2和MAT 1 -1-3在F. FpPPR 1基因缺失后，pseudograminearum的表达也下调，并通过实时定量PCR进行了验证。此外，21个基因编码的推定异核体不相容蛋白下调。突变体的黄色色素沉着与PKS 12簇基因的表达减少相关。综上所述，我们对FpPpr 1的研究结果表明，这种PPR蛋白在真菌的无性发育、异核体形成的调节、交配型和致病性中具有多种功能。假禾谷孢属
Fusarium crown rot (FCR) and Fusarium head blight (FHB) are caused by Fusarium pseudograminearum and are newly emerging diseases of wheat in China. In this study, we characterized FpPPR1, a gene that encodes a protein with 12 pentatricopeptide repeat (PPR) motifs. The radial growth rate of the ΔFpppr1 deletion mutant was significantly slower than the wild type strain WZ-8A on potato dextrose agar plates and exhibited significantly smaller colonies with sector mutations. The aerial mycelium of the mutant was almost absent in culture tubes. The ΔFpppr1 mutant was able to produce spores, but spores of abnormal size and altered conidium septum shape were produced with a significant reduction in sporulation compared to wild type. ΔFpppr1 failed to cause disease on wheat coleoptiles and barley leaves using mycelia plugs or spore suspensions. The mutant phenotypes were successfully restored to the wild type levels in complemented strains. FpPpr1-GFP signals in spores and mycelia predominantly overlapped with Mito-tracker signals, which substantiated the mitochondria targeting signal prediction of FpPpr1. RNAseq revealed significant transcriptional changes in the ΔFpppr1 mutant with 1,367 genes down-regulated and 1,333 genes up-regulated. NAD-binding proteins, thioredoxin, 2Fe-2S iron-sulfur cluster binding domain proteins, and cytochrome P450 genes were significantly down-regulated in ΔFpppr1, implying the dysfunction of mitochondria-mediated reductase redox stress in the mutant. The mating type idiomorphic alleles MAT1-1-1, MAT1-1-2, and MAT1-1-3 in F. pseudograminearum were also down-regulated after deletion of FpPPR1 and validated by real-time quantitative PCR. Additionally, 21 genes encoding putative heterokaryon incompatibility proteins were down-regulated. The yellow pigmentation of the mutant was correlated with reduced expression of PKS12 cluster genes. Taken together, our findings on FpPpr1 indicate that this PPR protein has multiple functions in fungal asexual development, regulation of heterokaryon formation, mating-type, and pathogenesis in F. pseudograminearum.