Nitroprusside-sensitive and insensitive guanylate cyclases in retinal rod outer segments.
Nitroprusside-sensitive and insensitive guanylate cyclases in retinal rod outer segments.
复制标题
视网膜杆外节中硝普钠敏感和不敏感的鸟苷酸环化酶。
DOI：
10.1016/0006-291x(92)91713-z
复制
发表时间：
1992
影响因子：
3.1
通讯作者：
Sitaramayya,A
中科院分区：
文献类型：
--
作者：
Margulis,A;Sharma,RK;Sitaramayya,A
视杆细胞外节的视网膜含有鸟苷酸环化酶活性的细胞质和膜组分。虽然细胞质中的活性是总活性的一小部分，但它被一氧化氮生成剂硝普钠高度活化。另一方面，膜鸟苷酸环化酶在溶解之前和之后都不受硝普钠的影响。因此，硝普钠或一氧化氮对感光细胞功能的影响应该是由胞质而不是膜鸟苷酸环化酶介导的。
Rod outer segments of retina contain guanylate cyclase activity both in the cytosol and membrane fractions. Though the activity in the cytosol is a small fraction of the total activity, it is highly activated by nitroprusside, a nitric oxide generating agent. The membrane guanylate cyclase on the other hand is unaffected by nitroprusside both before and after solubilization. The effects of nitroprusside or nitric oxide on photoreceptor function should therefore be mediated by the cytosolic and not the membrane guanylate cyclase.