Repair of Rat Sciatic Nerve Defects by Using Allogeneic Bone Marrow Mononuclear Cells Combined with Chitosan/Silk Fibroin Scaffold
Repair of Rat Sciatic Nerve Defects by Using Allogeneic Bone Marrow Mononuclear Cells Combined with Chitosan/Silk Fibroin Scaffold
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DOI：
10.3727/096368916x690494
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发表时间：
2016-05
影响因子：
3.3
通讯作者：
Min Yao;Yi Zhou;Chengbin Xue;Hechun Ren;Shengran Wang;Hui Zhu;Xingjian Gu;Xiaosong Gu;J. Gu
中科院分区：
文献类型：
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作者：
Min Yao;Yi Zhou;Chengbin Xue;Hechun Ren;Shengran Wang;Hui Zhu;Xingjian Gu;Xiaosong Gu;J. Gu
骨髓单核细胞（BM-MNCs）在许多疾病中的治疗益处已得到充分证实。为了将基于BM-MNC的细胞疗法推进到临床周围神经修复中，在这项研究中，我们开发了一种新的组织工程神经移植物（TENGs）设计，其由基于壳聚糖/丝素蛋白的神经支架和作为支持细胞的BM-MNC组成。这些TENG用于间置神经移植桥接大鼠坐骨神经缺损10 mm长。神经移植后的组织学和功能评估表明，我们开发的TENG实现的再生效果优于壳聚糖/丝素蛋白支架，并接近自体神经移植物。此外，我们使用绿色荧光蛋白标记的BM-MNCs来跟踪壳聚糖/丝素蛋白基神经支架内的细胞位置，并在坐骨神经再生的早期阶段跟踪细胞命运。结果表明，BM-MNCs在神经移植后至少可以存活2周，从而有助于初步了解BM-MNCs对轴突再生的有利作用机制。
The therapeutic benefits of bone marrow mononuclear cells (BM-MNCs) in many diseases have been well established. To advance BM-MNC-based cell therapy into the clinic for peripheral nerve repair, in this study we developed a new design of tissue-engineered nerve grafts (TENGs), which consist of a chitosan/fibroin-based nerve scaffold and BM-MNCs serving as support cells. These TENGs were used for interpositional nerve grafting to bridge a 10-mm-long sciatic nerve defect in rats. Histological and functional assessments after nerve grafting showed that regenerative outcomes achieved by our developed TENGs were better than those achieved by chitosan/silk fibroin scaffolds and were close to those achieved by autologous nerve grafts. In addition, we used green fluorescent protein-labeled BM-MNCs to track the cell location within the chitosan/fibroin-based nerve scaffold and trace the cell fate at an early stage of sciatic nerve regeneration. The result suggested that BM-MNCs could survive at least 2 weeks after nerve grafting, thus helping to gain a preliminary mechanistic insight into the favorable effects of BM-MNCs on axonal regrowth.