Role of confinement in the active self-organization of kinesin-driven microtubules
Role of confinement in the active self-organization of kinesin-driven microtubules
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限制在驱动蛋白驱动的微管主动自组织中的作用
DOI：
10.1016/j.snb.2017.03.006
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Kakugo Akira
中科院分区：
文献类型：
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作者：
Islam Md. Sirajul;Kuribayashi-Shigetomi Kaori;Kabir Arif Md. Rashedul;Inoue Daisuke;Sada Kazuki;Kakugo Akira
自组织是生物活性系统中最引人注目的现象之一。许多研究试图调查不同的参数，调节运动物体的自组织。最近的理论和分析方法表明，物理约束对运动物体的自组织具有调节作用。然而，关于限制的不同形状和大小如何影响运动物体的自组织的详细实验研究仍然缺乏。近年来，肌动蛋白/肌球蛋白、微管/驱动蛋白或微管/动力蛋白等生物分子马达系统已成为研究运动物体自组织行为的重要工具。本文采用微管/驱动蛋白运动系统的体外运动实验，研究了限制对微管自组织的形状和尺寸效应。MT由通过光刻法微图案化的玻璃表面上的脂质层限制。我们证明，形状和大小的限制在很大程度上影响了自组织的MT。在不同形状和大小的限制中，MT表现出不同的取向。这项工作清楚地揭示了物理限制如何影响MT的自组织，并将有助于理解限制对自然界中更复杂的生物活性系统的自组织的影响。
Self-organization is one of the most spectacular phenomena exhibited in the wide spectrum of biologically active systems. Many studies have attempted to investigate different parameters that regulate the self-organization of moving objects. Recent theoretical and analytical-based approaches have revealed that physical confinement has regulatory effect on the self-organization of moving objects. However, a detailed experimental study on how the varying shapes and sizes of the confinement affect the self-organization of moving objects is still lacking. Recently, biomolecular motor systems F-actin/myosin and microtubule/kinesin or microtubule/dynein have been promising to experimentally study the self-organization of moving objects. Here, we experimentally investigated the shape and size effect of confinement on the self-organization of microtubules (MTs) by employing thein vitromotility assay of MT/kinesin motor system. The MTs were confined by a lipid layer on a glass surface micro-patterned by photolithography. We demonstrated that shapes and sizes of the confinements largely influenced the self-organization of MTs. The MTs showed distinct orientations in different shapes and sizes of the confinements. This work clearly unveiled how physical confinement influences the self-organization of MTs and would help understand the effect of confinement on the self-organization of more complex biologically active systems in nature.