Rapid determination of 2,4-diaminopyrimidine residues through sample pretreatment using immunomagnetic bead purification along with HPLC-UV
Rapid determination of 2,4-diaminopyrimidine residues through sample pretreatment using immunomagnetic bead purification along with HPLC-UV
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使用免疫磁珠纯化和 HPLC-UV 进行样品预处理，快速测定 2,4-二氨基嘧啶残留物
DOI：
10.1016/j.foodchem.2021.131835
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发表时间：
2022-01-05
期刊：
影响因子：
8.8
通讯作者：
Zhang，Xiya
中科院分区：
文献类型：
--
作者：
Wang，Liye;Niu，Jiangxiu;Zhang，Xiya
为了减少基质干扰，同时检测猪、牛、鸡、鱼肌肉中多种同源化合物(甲氧苄啶（TMP）、双苯胺（DVD）、奥美托啶（OMP）、巴喹啶（BQP）和阿地托啶（ADP），建立了基于免疫磁珠（IMB）的HPLC-UV样品纯化预处理方法。制备了一种广谱单克隆抗体（mAb，命名为14C6），并采用活性酯法与羧酸功能化磁性纳米颗粒偶联，得到用于样品纯化的IMBs。根据提取效率对提取溶剂进行了优化。5种分析物（10 ~ 200 μg/kg）均具有良好的线性关系，定量限和定量限分别为5和10 μg/kg。平均加样回收率为62.5% ~ 76.9%，变异系数<12.2%。与基于固相萃取柱的传统方法相比，IMB方法具有更好的样品纯化和富集效果。因此，基于imb的样品纯化是测定可食用动物组织中2,4-二氨基嘧啶残留的有效工具。
To reduce matrix interference and realize simultaneous detection of multiple homologous compounds (trimethoprim (TMP), diaveridine (DVD), ormetoprim (OMP), baquiloprim (BQP), and aditoprim (ADP) in pig, cattle, chicken, and fish muscles), an immunomagnetic bead (IMB)-based sample purification pretreatment with HPLC–UV was developed. A broad-spectrum monoclonal antibody (mAb, named 14C6) was prepared and conjugated with carboxylic-acid-functionalized magnetic nanoparticles using the active ester method to obtain IMBs for sample purification. The extraction solvent was optimized based on the extraction efficiency. Good linearity was observed for all the five analytes (10–200 μg/kg) with the LOD and LOQ of 5 and 10 μg/kg, respectively. The mean recoveries ranged from 62.5% to 76.9%, while the coefficient of variation was <12.2%. The IMB method afforded greater sample purification and enrichment than those achieved with the SPE column-based conventional method. Hence, the IMB-based sample purification is a useful tool to determine 2,4-diaminopyrimidine residues in edible animal tissues.