DNA-Encoded Raman-Active Anisotropic Nanoparticles for microRNA Detection
DNA-Encoded Raman-Active Anisotropic Nanoparticles for microRNA Detection
复制标题
用于 microRNA 检测的 DNA 编码拉曼活性各向异性纳米颗粒
DOI：
10.1021/acs.analchem.7b01861
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发表时间：
2017
影响因子：
7.4
通讯作者：
Pei Hao
中科院分区：
文献类型：
--
作者：
Qi Lin;Xiao Mingshu;Wang Xiwei;Wang Cheng;Wang Lihua;Song Shiping;Qu Xiangmeng;Li Li;Shi Jiye;Pei Hao
由于microRNA（miRNA）在基础生物学研究和诊断应用中的重要性，开发高灵敏度和高选择性的microRNA（miRNA）检测方法受到了极大的关注。在这项工作中，我们开发了DNA编码的拉曼活性各向异性纳米颗粒修饰的折纸分析装置（oPAD），用于快速，高灵敏度和特异性的miRNA检测。利用10-mer oligo-A、-T、-C和-G介导Ag立方体种子生长成不同形貌的Ag纳米颗粒（AgNPs），制备了具有拉曼活性的各向异性纳米颗粒。所得的银纳米粒子进一步编码的DNA探针作为有效的表面增强拉曼散射（Sers）探针。然后，分析装置被制造在一片装载有Sers探针的纸基基底上，并基于折纸的原理组装。目标分析物的加入通过目标依赖性的序列特异性DNA杂交组装放大了DNA编码的AgNP上的拉曼信号。这种简单且低成本的分析装置是通用的，适用于各种miRNA，允许检测灵敏度低至1 pM，测定时间在15分钟内，因此在护理点诊断中具有很好的应用前景。
The development of highly sensitive and selective methods for the detection of microRNA (miRNA) has attracted tremendous attention because of its importance in fundamental biological studies and diagnostic applications. In this work, we develop DNA-encoded Raman-active anisotropic nanoparticles modified origami paper analytical devices (oPADs) for rapid, highly sensitive, and specific miRNA detection. The Raman-active anisotropic nanoparticles were prepared using 10-mer oligo-A, -T, -C, and -G to mediate the growth of Ag cubic seeds into Ag nanoparticles (AgNPs) with different morphologies. The resulting AgNPs were further encoded with DNA probes to serve as effective surface-enhanced Raman scattering (SERS) probes. The analytical device was then fabricated on a single piece of SERS probes loaded paper-based substrate and assembled based on the principles of origami. The addition of the target analyte amplifies the Raman signals on DNA-encoded AgNPs through a target-dependent, sequence specific DNA hybridization assembly. This simple and low-cost analytical device is generic and applicable to a variety of miRNAs, allowing detection sensitivity down to 1 pM and assay time within 15 min, and therefore holds promising applications in point-of-care diagnostics.