MOLECULAR-WEIGHT AND VALENCE OF CELL-SURFACE RECEPTOR FOR IMMUNOGLOBULIN-E
MOLECULAR-WEIGHT AND VALENCE OF CELL-SURFACE RECEPTOR FOR IMMUNOGLOBULIN-E
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DOI：
10.1073/pnas.74.3.869
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发表时间：
1977-01-01
影响因子：
11.1
通讯作者：
METZGER, H
中科院分区：
文献类型：
--
作者：
NEWMAN, SA;ROSSI, G;METZGER, H
The MW of the active solubilized [rat basophilic leukocyte] cell-surface receptor for immunoglobulin (Ig)E was measured in nonionic detergent. The diffusion coefficient was estimated by gel filtration, the partial specific volume was estimated from the differential sedimentation in sucrose gradients prepared from H2O and D2O [deuterium oxide], and the sedimentation constant was estimated from the same centrifugation experiments. The receptor has an apparent MW of 130,000. Its high partial specific volume (0.81 cm3/g) suggests that bound detergent contributes significantly to the mass. The MW of the receptor-IgE complex and of unbound IgE (determined similarly) were 310,000 and 200,000, respectively, showing that the receptor is univalent. The implications of these results for the subunit structure of the receptor, receptor-membrane integration, and a possible mechanism of receptor triggering are discussed.
