Laser capture microdissection: Big data from small samples.
Laser capture microdissection: Big data from small samples.
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DOI：
10.14670/hh-11-622
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发表时间：
2015-11
影响因子：
2
通讯作者：
Roy S
中科院分区：
文献类型：
--
作者：
Datta S;Malhotra L;Dickerson R;Chaffee S;Sen CK;Roy S
任何组织都是由空间分布的细胞类型的异质混合组成的。在对任何（病理）生理线索的反应中，任何给定组织中每种细胞类型的反应可能是唯一的，并且不能在细胞类型和空间坐标中均质化。例如，对心肌梗塞的反应，一方面，心肌细胞和心脏组织的成纤维细胞的反应不同。另一方面，梗死核心的肌细胞与梗死周围区的肌细胞反应不同。因此，分离纯靶细胞是对参与疾病进展的细胞进行分子分析的重要和必要步骤。激光捕获显微解剖技术（LCM）能够在显微镜下快速精确地获得纯靶细胞亚群，甚至单个细胞，成功地解决了分子分析中的组织异质性问题。本文综述了LCM技术的基本原理、优点和局限性及其在蛋白质组学、基因组学和转录组学等领域的下游应用。凭借强大的技术和适当的应用，这项技术为细胞生物学提供了前所未有的见解，这些细胞生长在自然组织栖息地，而不是在人工培养皿条件下培养。
Any tissue is made up of a heterogeneous mix of spatially distributed cell types. In response to any (patho) physiological cue, responses of each cell type in any given tissue may be unique and cannot be homogenized across cell-types and spatial co-ordinates. For example, in response to myocardial infarction, on one hand myocytes and fibroblasts of the heart tissue respond differently. On the other hand, myocytes in the infarct core respond differently compared to those in the peri-infarct zone. Therefore, isolation of pure targeted cells is an important and essential step for the molecular analysis of cells involved say in the progression of disease. Laser capture microdissection (LCM) is powerful to obtain a pure targeted cell subgroup, or even a single cell, quickly and precisely under the microscope, successfully tackling the problem of tissue heterogeneity in molecular analysis. This review presents an overview of LCM technology, the principles, advantages and limitations and its down-stream applications in the fields of proteomics, genomics and transcriptomics. With powerful technologies and appropriate applications, this technique provides unprecedented insights into cell biology from cells grown in their natural tissue habitat as opposed to those cultured in artificial petri dish conditions.