Blood culture systems: rapid detection--how and why?
Blood culture systems: rapid detection--how and why?
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DOI：
10.1016/s0924-8579(09)70559-x
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发表时间：
2009-01-01
影响因子：
10.8
通讯作者：
Sambri, Vittorio
中科院分区：
文献类型：
--
作者：
Gaibani, Paolo;Rossini, Giada;Sambri, Vittorio
血流感染与高发病率和死亡率相关。血培养被认为是诊断中最重要的工具。在这项研究中，我们将标准血培养与两种不同的分子技术（Prove-it Sepsis 和 LightCycler SeptiFast M）进行比较，以检测脓毒症患者血液中的细菌种类和真菌。两种方法均与血培养获得的结果显着一致。这些研究的结果表明，LightCycler SeptiFast 和 Prove-it Sepsis 检测均可用于菌血症和血流感染的快速、特异性诊断。然而，重要的是要强调分子方法不能取代血培养，因为后者可以评估分离微生物的抗菌敏感性。
Bloodstream infections are associated with high morbidity and mortality. Blood culture is considered to be the most important tool in their diagnosis. In this study we compared standard blood culture with two different molecular techniques (Prove-it Sepsis and LightCycler SeptiFast M) to detect bacterial species and fungi in the blood of septic patients. Both methods showed significant agreement with the results obtained by blood culture. The results of these studies suggest that both the LightCycler SeptiFast and Prove-it Sepsis tests can be used for the rapid and specific diagnosis of bacteraemia and bloodstream infections. It is important, however, to emphasize that molecular methods cannot replace blood cultures, as the latter allow the evaluation of the antimicrobial susceptibility of isolated microbes.