A comparative study of the early osmotic, ionic, redox and hormonal signaling response in leaves and roots of two halophytes and a glycophyte to salinity
A comparative study of the early osmotic, ionic, redox and hormonal signaling response in leaves and roots of two halophytes and a glycophyte to salinity
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DOI：
10.1007/s00425-014-2154-7
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发表时间：
2014-12-01
期刊：
影响因子：
4.3
通讯作者：
Munne-Bosch, Sergi
中科院分区：
文献类型：
--
作者：
Ellouzi, Hasna;Ben Hamed, Karim;Munne-Bosch, Sergi
盐胁迫是影响自然生态系统中植物生长和生产力的重要非生物胁迫因子之一。在这项研究中，我们旨在确定耐盐和盐敏感物种在叶片和根的早期(72h内)盐胁迫反应中的可能差异。为此，我们对三种十字花科植物，即两种盐生植物Cakile maritima和Thellungiella salsugina和一种糖类植物拟南芥进行了短期盐胁迫(400 MMNaC L)。结果表明，盐生植物表现出不同的渗透和离子反应，伴随着早期和瞬时的氧化爆发，其特征是叶片和根中过氧化氢水平的增加和随后的抗氧化防御的激活。此外，在盐胁迫下，盐生植物叶片和根中脱落酸、茉莉酸(JA)和ACC(乙烯前体氨基环丙烷-1-羧酸)的积累均高于海棠。此外，在盐胁迫下，盐生植物在叶片和根中都表现出乙烯反应因子1(ERF1)的表达增强，这是JA和乙烯信号转导通路的汇聚节点。综上所述，我们的研究结果表明，与甜菜相比，盐生植物马利玛和盐碱蓬不仅在叶片中，而且在根中都经历了早期的氧化爆发，抗氧化防御和激素反应都得到了改善。这种差异信号反应至少部分地汇聚为地上和地下组织中ERF1表达的增加，似乎至少部分地支持了这两种所研究的盐生植物对盐胁迫的耐受性增强。
Salt stress is one of the most important abiotic stress factors affecting plant growth and productivity in natural ecosystems. In this study, we aimed at determining possible differences between salt tolerant and salt sensitive species in early (within 72 h) salt stress response in leaves and roots. To this purpose, we subjected three Brassicaceae species, namely two halophytes-Cakile maritima and Thellungiella salsuginea-and a glycophyte-Arabidopsis thaliana- to short-term salt stress (400 mM NaCl). The results indicate that the halophytes showed a differential osmotic and ionic response together with an early and transient oxidative burst, which was characterized by enhanced hydrogen peroxide levels and subsequent activation of antioxidant defenses in both leaves and roots. In addition, the halophytes displayed enhanced accumulation of abscisic acid, jasmonic acid (JA) and ACC (aminocyclopropane-1-carboxylic acid, the precursor of ethylene) in leaves and roots, as compared to A. thaliana under salt stress. Moreover, the halophytes showed enhanced expression of ethylene response factor1 (ERF1), the convergence node of the JA and ethylene signaling pathways in both leaves and roots upon exposure to salt stress. In conclusion, we show that the halophytes C. maritima and T. salsuginea experience an early oxidative burst, improved antioxidant defenses and hormonal response not only in leaves but also in roots, in comparison to the glycophyte A. thaliana. This differential signaling response converging, at least in part, into increased ERF1 expression in both above- and underground tissues seems to underlay, at least in part, the enhanced tolerance of the two studied halophytes to salt stress.