C-13 NUCLEAR-MAGNETIC-RESONANCE STUDIES OF PSEUDOMONAS-PUTIDA FATTY-ACID METABOLIC ROUTES INVOLVED IN POLY(3-HYDROXYALKANOATE) SYNTHESIS
C-13 NUCLEAR-MAGNETIC-RESONANCE STUDIES OF PSEUDOMONAS-PUTIDA FATTY-ACID METABOLIC ROUTES INVOLVED IN POLY(3-HYDROXYALKANOATE) SYNTHESIS
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DOI：
10.1128/jb.176.6.1661-1666.1994
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发表时间：
1994-03-01
影响因子：
3.2
通讯作者：
EGGINK, G
中科院分区：
文献类型：
--
作者：
HUIJBERTS, GNM;DERIJK, TC;EGGINK, G
采用C-13核磁共振谱、气相色谱、气相色谱-质谱联用技术研究了不同碳源下恶臭假单胞菌KT2442中聚（3-羟基烷酸酯）（PHAs）的形成过程。用[1-C-13]癸酸酯证实了β -氧化与PHA形成的关系。由[1-C-13]醋酸酯合成的相芳烃中的标记模式对应于通过脂肪酸生物合成重新生成相芳烃。对脂肪酸代谢途径特异性抑制剂的研究表明，β -氧化和新生脂肪酸生物合成在PHA形成中独立起作用。对[1-C-13]己酸酯衍生的PHA的分析表明，两种脂肪酸代谢途径可以同时作用于PHA的合成。此外，有证据表明，在中链脂肪酸生长过程中，PHA前体可以通过这些脂肪酸与乙酰辅酶A分子的延伸产生，可能是通过3-酮硫酶的反向作用。
The formation of poly(3-hydroxyalkanoates) (PHAs) in Pseudomonas putida KT2442 from various carbon sources was studied by C-13 nuclear magnetic resonance spectroscopy, gas chromatography, and gas chromatography-mass spectroscopy. By using [1-C-13]decanoate, the relation between beta-oxidation and PHA formation was confirmed. The labeling pattern in PHAs synthesized from [1-C-13]acetate corresponded to the formation of PHAs via de novo fatty acid biosynthesis. Studies with specific inhibitors of the fatty acid metabolic pathways demonstrated that beta-oxidation and de novo fatty acid biosynthesis function independently in PHA formation. Analysis of PHAs derived from [1-C-13]hexanoate showed that both fatty acid metabolic routes can function simultaneously in the synthesis of PHA. Furthermore, evidence is presented that during growth on medium-chain-length fatty acids, PHA precursors can be generated by elongation of these fatty acids with an acetyl coenzyme A molecule, presumably by a reverse action of 3-ketothiolase.