Dietary zinc deficiency increases uroguanylin accumulation in rat kidney.
Dietary zinc deficiency increases uroguanylin accumulation in rat kidney.
复制标题
膳食锌缺乏会增加大鼠肾脏中尿鸟苷蛋白的积累。
DOI：
10.1046/j.1523-1755.2001.0590041424.x
复制
发表时间：
2001
影响因子：
19.6
通讯作者：
Cousins,RJ
中科院分区：
文献类型：
--
作者：
Cui,L;Blanchard,RK;Cousins,RJ
膳食锌缺乏增加尿鸟苷素积累在rat kidney.BackgroundZn2+缺乏在人类产生的分泌性腹泻是纠正锌补充剂。在大鼠中，差异mRNA显示分析表明，肠道尿鸟苷素基因表达增加锌缺乏。可能存在涉及肠尿鸟苷素和肾脏的内分泌轴。因此，我们进行了这项研究，以检查是否锌缺乏会影响尿鸟苷素在肾脏中的表达rates.MethodsA纯化的饮食，缺乏或足够的锌含量，喂给大鼠。原位杂交显示尿鸟苷素原mRNA定位于肾脏，免疫组化显示尿鸟苷素原/尿鸟苷素肽定位于肾脏。通过Western blotting和狭缝blotting analysis.ResultsIn原位杂交表明，preprouroguanylin mRNA表达细胞定位在近端小管，主要限于皮质-髓质交界处。锌缺乏没有改变mRNA的丰度或分布。使用尿鸟苷肽特异性、亲和纯化的抗体进行免疫组织化学，证明免疫反应性尿鸟苷肽定位于相同的细胞，但在缺锌大鼠中染色更强。肾脏提取物的蛋白质印迹分析表明，有没有丰富的尿鸟苷素锌充足和缺乏大鼠之间的差异。然而，狭缝印迹分析表明，丰富的低分子量的免疫反应性肽，推测uroguanylin，是较高的锌缺乏rates.ConclusionThe结果表明，生产的prouroguanylin的肾脏，在对比肠，是不受饮食锌摄入量，但肾提取物中较高量的尿鸟苷素可能反映了从体循环获得的激素的肾处理。
Dietary zinc deficiency increases uroguanylin accumulation in rat kidney.BackgroundZinc deficiency in humans produces a secretory diarrhea that is corrected by zinc supplementation. In rats, differential mRNA display analysis has shown that intestinal uroguanylin gene expression is increased in zinc deficiency. An endocrine axis involving intestinal uroguanylin and the kidney may exist. Therefore, we conducted this study to examine whether zinc deficiency would affect uroguanylin expression in the kidney of rats.MethodsA purified diet, deficient or adequate in zinc content, was fed to rats. Preprouroguanylin mRNA was localized in kidney by in situ hybridization, and prouroguanylin/uroguanylin peptides were localized in the kidney by immunohistochemistry. Abundance was measured by Western blotting and slot blotting analyses.ResultsIn situ hybridization demonstrated that preprouroguanylin mRNA-expressing cells were localized in the proximal tubules, being primarily limited to the cortical-medullary junction. Zinc deficiency did not alter the abundance or distribution of the mRNA. Immunohistochemistry, using a uroguanylin peptide-specific, affinity-purified antibody, demonstrated that immunoreactive uroguanylin peptide was localized to the same cells but that the staining was stronger in zinc-deficient rats. Western blotting analysis of kidney extracts showed that there was no difference in abundance of prouroguanylin between zinc adequate and deficient rats. However, slot blotting analysis demonstrated that the abundance of a low molecular weight immunoreactive peptide, presumably uroguanylin, was higher in extracts of zinc-deficient rats.ConclusionThe results suggest that production of prouroguanylin by the kidney, in contrast to the intestine, is not influenced by dietary zinc intake, but that higher amounts of uroguanylin in kidney extracts may reflect renal processing of the hormone obtained from the systemic circulation.