A missense mutation of the gene for Na+,K+-ATPase α-subunit causes abnormal feeding behavior in Caenorhabditis elegans
A missense mutation of the gene for Na+,K+-ATPase α-subunit causes abnormal feeding behavior in Caenorhabditis elegans
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DOI：
10.1006/bbrc.1998.8981
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发表时间：
1998-07-30
影响因子：
3.1
通讯作者：
Ohta, H
中科院分区：
文献类型：
--
作者：
Shima, Y;Tada, Y;Ohta, H
本文研究了一株行为突变型C.发现秀丽线虫的感染率约为野生型的三分之一。Na+，K+-ATP酶α亚基的mRNA和多肽水平与野生型相当，但磷酸化中间体的水平比野生型低80%。在参与磷酸化中间体形成的α-亚基的高度保守区域中发现了单个预测的氨基酸置换（Leu至Phe）。该突变体的异常摄食行为可能与该错义突变有关。(C)北京：科学出版社.
The Na+,K+-ATPase activity of membranes from a behavioral mutant of C. elegans was found to be about one-third that of the wild-type. The levels of mRNA and polypeptide of Na+,K+-ATPase alpha-subunit in the mutant were as high as those in the wild-type, but the level of the phosphorylated intermediate of the Na+,K+-ATPase in the mutant worm was 80% lower than that in the wild-type. A single predicted amino acid replacement (Leu to Phe) was found in a highly conserved region in the alpha-subunit that is involved in the formation of phosphorylated intermediate. The abnormal feeding behavior of the mutant worm may be attributed to this missense mutation. (C) 1998 Academic Press.