Spatial extent of CBF response during whisker stimulation using trial averaged laser Doppler imaging.
Spatial extent of CBF response during whisker stimulation using trial averaged laser Doppler imaging.
复制标题
使用试验平均激光多普勒成像观察晶须刺激期间 CBF 响应的空间范围。
DOI：
10.1016/j.brainres.2006.02.114
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Biswal,BharatB
中科院分区：
文献类型：
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作者：
Kannurpatti,SridharS;Biswal,BharatB
用高分辨率激光多普勒(LD)成像技术研究了乌拉坦麻醉大鼠对多次胡须刺激的激活反应的空间模式。代表脑血流(CBF)反应的LD流量变化从单个试验或在平均多个类似试验后进行分析。在单个试验中，脑血流量的变化主要是在具有低基线流量值的像素(微血管)上观察到的，其中包括直径400-800μm的弥漫性环形激活图案，类似于在大鼠躯体感觉皮质IV层建立的单个桶的组织尺寸。每一次试验的整体激活模式差异很大(只有大约9%-10%的重叠)；然而，在所有大鼠内部和跨所有大鼠的每一次单独试验中，激活的扩散环形模式都是可重现的。通过试算平均值，激活的外边界没有明显增加，但激活的弥漫圆形模式内的激活像素数量增加。进一步的活跃像素主要出现在活动的弥散圆形区域内，试算平均表明隔区和桶状区域有明显的CBF反应，桶状核的Ld信噪比较小。
The spatial pattern of activation in response to multiple whisker stimulation was studied using high-resolution laser Doppler (LD) imaging in urethane-anesthetized rats. LD flux change representing cerebral blood flow (CBF) responses were analyzed from a single trial or after averaging a number of similar trials. CBF change in a single trial was observed predominantly over pixels having low baseline flux values (microvessels), which included diffuse circular patterns of activation 400–800 μm in diameter similar to the histological dimensions of individual barrels established in the layer IV of the rat somatosensory cortex. The overall activation pattern varied considerably between each trial (only about 9–10% overlap); however, the diffuse circular pattern of activation was reproducible in every single trial within and across all rats. With trial averaging, no significant increase was observed in the outer boundary of activation, but the number of activated pixels increased within the diffuse circular patterns of activation. Emergence of further active pixels primarily within the diffuse circular regions of activity with trial averaging indicates distinct CBF responses in the septal and barrel regions, with a lesser LD signal to noise ratio in the barrel core.