EVIDENCE THAT THE CENTRALLY AND PERIPHERALLY LOCATED CELLS IN THE MURINE EPIDERMAL PROLIFERATIVE UNIT ARE 2 DISTINCT CELL-POPULATIONS
EVIDENCE THAT THE CENTRALLY AND PERIPHERALLY LOCATED CELLS IN THE MURINE EPIDERMAL PROLIFERATIVE UNIT ARE 2 DISTINCT CELL-POPULATIONS
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DOI：
10.1111/1523-1747.ep12265358
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发表时间：
1985-01-01
影响因子：
6.5
通讯作者：
SLAGA, TJ
中科院分区：
文献类型：
--
作者：
MORRIS, RJ;FISCHER, SM;SLAGA, TJ
对成年senar小鼠背表皮的[3H]胸腺嘧啶标记保留和成熟基底细胞进行了表征，并比较了它们对局部应用肿瘤启动子12- o - tetradecanoylphorpol -13-acetate （TPA）的早期细胞动力学反应。全表皮和横切面放射自显影显示，每6小时注射[3H]胸腺嘧啶，持续1周，标记95%的基底核，包括表皮增殖单位中心区域的核。1个月后，标示指数降至2%；90%的标记保留细胞位于增殖单位中央基底上柱的核直径范围内。给小鼠注射2。标记后1小时，在处理后22小时内发现有丝分裂保留标记的细胞。在28小时的实验期间，75%的标记保留细胞留在基底层。胸苷[3H]脉冲1小时后的基础标记指数为5%。85%的标记细胞位于增殖单位的周围。脉冲标记4天后，大部分原标记的细胞已经分裂，尽管垂直截面显示92%的细胞仍在基底层。给小鼠TPA处理第4天，标记细胞很少出现有丝分裂。相反，约60%的标记细胞移位到基底上层。两类表皮基底细胞对TPA的早期反应明显不同；保留标记的细胞增殖，大多数成熟细胞继续分化。
The [3H]thymidine label-retaining and the maturing classes of basal cells from the dorsal epidermis of adult SENCAR mice were characterized and their early cellular kinetic responses to topical application of the tumor promoter, 12-O-tetradecanoylphorbol-13-acetate (TPA) compared. Autoradiography of epidermal whole mounts and cross sections demonstrated that injection of [3H]thymidine every 6 h for 1 wk labeled 95% of the basal nuclei, including those in the central region of the epidermal proliferative units. One month later, the labeling index was reduced to 2%; 90% of the label-retaining cells were within a nuclear diameter of the central suprabasal column of the proliferative units. When mice were treated with 2 .mu.g of TPA 1 mo. after labeling, mitotic label-retaining cells were found within 22 h after treatment. Seventy-five percent of the label-retaining cells remained on the basal layer through the 28-h expeirmental period. The basal labeling index following a 1-h pulse of [3H]thymidine was 5%. Eighty-five percent of the labeled cells were found in the periphery of the proliferative units. By 4 days after pulse labeling, most of the originally labeled cells had divided, although vertical cross sections indicated that 92% remained on the basal layer. When mice were treated with TPA on day 4, labeled cells were rarely found in mitosis. Instead, about 60% of the labeled cells were displaced to the suprabasal layers. The 2 classes of epidermal basal cells evidently have different early responses to TPA treatment; the label-retaining cells proliferate and most of the maturing cells continue to differentiate.