NUTRITION AND METABOLISM OF MARINE BACTERIA .15. RELATION OF NA+-ACTIVATED TRANSPORT TO NA+ REQUIREMENT OF A MARINE PSEUDOMONAD FOR GROWTH
NUTRITION AND METABOLISM OF MARINE BACTERIA .15. RELATION OF NA+-ACTIVATED TRANSPORT TO NA+ REQUIREMENT OF A MARINE PSEUDOMONAD FOR GROWTH
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DOI：
10.1128/jb.92.1.63-71.1966
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发表时间：
1966-01-01
影响因子：
3.2
通讯作者：
MACLEOD, RA
中科院分区：
文献类型：
--
作者：
DRAPEAU, GR;MATULA, TI;MACLEOD, RA
A marine pseudomonad was found to require 50 to 100 m[image] Na+ for maximal rate of oxidation of D-galactose and for the transport of D-fucose-H3 into the cells. The same organism required 150 to 200 m[image] Na+ for the oxidation of L-alanine and for the transport of [alpha]-aminoisobutyric acid-C14 (AIB-C14) into the cells. Competition studies indicated that D-galactose and D-fucose on the one hand and L-alanine and AIB on the other shared common carriers for transporting the compounds into the cells. This parallelism in Na+ response for oxidation and transport extended to growth when L-alanine was the sole carbon source in the medium. When D-galactose was the sole carbon source, an amount of Na+ equal to that with L-alanine was needed. KCN and dinitrophenol but not ouabain inhibited the uptake of AIB-C14 by the cells. K+ in addition to Na+ was required for transport, and both Mg++ and either Cl- or Br- were stimulatory. Photobacterium fischeri was also found to require Na+ specifically for the uptake of AIB-C14 by the cells.
