Differential effects of eicosapentaenoic acid and docosahexaenoic acid on vascular reactivity of the forearm microcirculation in hyperlipidemic, overweight men
Differential effects of eicosapentaenoic acid and docosahexaenoic acid on vascular reactivity of the forearm microcirculation in hyperlipidemic, overweight men
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DOI：
10.1161/01.cir.102.11.1264
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发表时间：
2000-09-12
期刊：
影响因子：
37.8
通讯作者：
Beilin, LJ
中科院分区：
文献类型：
--
作者：
Mori, TA;Watts, GF;Beilin, LJ
背景-最近的证据支持二十碳五烯酸（EPA）和二十二碳六烯酸（DHA），海洋来源的2种主要ω 3脂肪酸，对人类血压和成年自发性高血压大鼠血管反应性的不同影响。我们研究了纯化的EPA或DHA对超重高血脂症男性前臂血管反应性的影响的可能差异，这可能有助于鱼油的降压作用。方法和结果-采用双盲，安慰剂对照平行设计试验，59名超重，轻度高血脂症男性随机接受4 g/d纯化的EPA，DHA，或橄榄油（安慰剂）胶囊，同时继续他们的日常饮食6周。采用静脉阻断法、应变式容积描记法测定乙酰胆碱序贯动脉灌注时前臂血流量（7.5、15和30 μ g/min），硝普钠（1.5、3和10 μ g/min），去甲肾上腺素（10、20和40 ng/min），单次输注N-G-单甲基-L-精氨酸（L-NMMA）（1毫克/分钟），并共同输注乙酰胆碱（7.5，15，和30微克/分钟）和L-NMMA。完成研究的56例受试者中有40例接受了FBF测量。补充EPA后，血浆磷脂EPA水平显著升高（P < 0.0001），DHA组成随补充DHA而增加（P < 0.0001）。相对于安慰剂，DHA，而不是EPA，补充显着改善FBF响应乙酰胆碱输注（P = 0.040）和乙酰胆碱与L-NMMA的共输注（P = 0.040）。L-NMMA单独输注未显示组间差异。DHA显著增强了对硝普钠的舒张反应（P < 0.0001），减弱了对去甲肾上腺素的收缩反应（P = 0.017）。内皮非依赖性机制的改善似乎是主要的，可能有助于在人体中观察到的DHA与EPA相比的选择性降压作用。
Background-Recent evidence supports differential effects of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), the 2 major omega 3 fatty acids of marine origin, on blood pressure in humans and vascular reactivity in adult spontaneously hypertensive rats. We investigated possible differences in the effects of purified EPA or DHA on forearm vascular reactivity in overweight hyperlipidemic men that might contribute to the blood pressure-lowering effects of fish oils.Methods and Results-With a double-blind, placebo-controlled trial of parallel design, 59 overweight, mildly hyperlipidemic men were randomized to receive 4 g/d purified EPA, DHA, or olive oil (placebo) capsules while continuing their usual diets for 6 weeks. Forearm blood flow (FBF) was measured with venous occlusion, strain-gauge plethysmography during the sequential intra-arterial administration of acetylcholine (7.5, 15, and 30 mu g/min), sodium nitroprusside (1.5, 3, and 10 mu g/min), norepinephrine (10, 20, and 40 ng/min), a single-dose infusion of N-G-monomethyl-L-arginine (L-NMMA) (1 mg/min), and coinfusion of acetylcholine (7.5, 15, and 30 mu g/min) and L-NMMA. Forty of the 56 subjects who completed the study underwent FBF measurements. Plasma phospholipid EPA levels increased significantly (P < 0.0001) after supplementation with EPA, and DHA composition increased with DHA supplementation (P < 0.0001). Relative to placebo, DHA, but not EPA, supplementation significantly improved FBF in response to acetylcholine infusion (P = 0.040) and coinfusion of acetylcholine with L-NMMA (P = 0.040). Infusion of L-NMMA alone showed no group differences. DHA significantly enhanced dilatory responses to sodium nitroprusside (P < 0.0001) and attenuated constrictor responses to norepinephrine (P = 0.017).Conclusions-Relative to placebo, DHA, but not EPA, enhances vasodilator mechanisms and attenuates constrictor responses in the forearm microcirculation. Improvements in endothelium-independent mechanisms appear to be predominant and may contribute to the selective blood pressure-lowering effect observed with DHA compared with EPA in humans.