BIOCHEMICAL COMPLEMENTATION WITH RNA IN THE XENOPUS OOCYTE - A SMALL RNA IS REQUIRED FOR THE GENERATION OF 3' HISTONE MESSENGER-RNA TERMINI
BIOCHEMICAL COMPLEMENTATION WITH RNA IN THE XENOPUS OOCYTE - A SMALL RNA IS REQUIRED FOR THE GENERATION OF 3' HISTONE MESSENGER-RNA TERMINI
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DOI：
10.1016/0092-8674(83)90539-1
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发表时间：
1983-01-01
期刊：
影响因子：
64.5
通讯作者：
BIRNSTIEL, ML
中科院分区：
文献类型：
--
作者：
GALLI, G;HOFSTETTER, H;BIRNSTIEL, ML
当克隆的海胆Psammechinus miliaris的h22组蛋白基因重复单位被注射到青蛙卵母细胞核中时，转录忠实地在H3基因的启动子区域启动，但是转录终止或RNA加工以产生真正的H3 mRNA 3“末端是非常低效的。在注射组蛋白基因重复单元前1天，将小海胆聚（A）- RNA注射到卵母细胞细胞质中，可以增强3“末端的产生。该RNA是. apprx。60个核苷酸的长度，并出现在Psammechinus和Paracentrotus的胚胎相对较低的丰度。从细胞核的DEAE组分中提取的RNA，以前显示为补充H3基因表达中的损伤，也导致H3 mRNA的真实3“末端的出现。从12 S终止因子提取的RNA包括与来自poly（A）- RNA的60个核苷酸RNA共迁移的RNA种类。12 S核终止组分实际上是一种小的核RNP [核糖核蛋白]。
When the cloned h22 histone gene repeat unit of the sea urchin Psammechinus miliaris is injected into the frog oocyte nucleus, transcription initiates faithfully in the promoter region of the H3 gene, but transcription termination or RNA processing to yield genuine H3 mRNA 3'' ends is very inefficient. The generation of 3'' ends can be enhanced by the injection of a small sea urchin poly (A)- RNA into the oocyte cytoplasm 1 day prior to injection of the histone gene repeat unit. This RNA is .apprx. 60 nucleotides in length and appears to be present in relatively low abundance in embryos of both Psammechinus and Paracentrotus. RNA extracted from a nuclear DEAE fraction, previously shown to complement the lesion in H3 gene expression, also causes the appearance of true 3'' ends of H3 mRNA. RNA extracted from 12S termination factor includes an RNA species comigrating with the 60 nucleotide RNA from poly (A)- RNA. The 12S nuclear termination component is in fact a small nuclear RNP [ribonucleoprotein].