5-azacytidine induces chromosomal breakage in the root tips of wheat carrying the cuckoo chromosome 4SL from Aegilops sharonensis
5-azacytidine induces chromosomal breakage in the root tips of wheat carrying the cuckoo chromosome 4SL from Aegilops sharonensis
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DOI：
10.1046/j.1365-2540.2001.00931.x
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发表时间：
2001-10-01
期刊：
影响因子：
3.8
通讯作者：
Parker, JS
中科院分区：
文献类型：
--
作者：
de las Heras, JI;King, IP;Parker, JS
来自沙仑山羊草的杜鹃染色体4S(L)在通过杂交引入普通小麦、普通小麦时优先传递，4S(L)中携带的杀配子(Gc)因子诱导不含它们的减数孢子中的染色体断裂，确保其传递给后代。在小麦 4S(L) 附加系的早期胚囊发育过程中也可以观察到染色体断裂和断裂-融合-桥 (BFB) 循环。通常导致随后的后代中出现双着丝粒染色体。然而。负责诱导初级染色体断裂的过程似乎仅发生在胚胎和胚乳的初始分裂期间。存在 4S(L) 染色体。用低甲基化剂 5-氮杂胞苷处理会诱导根尖染色体断裂。这表明染色体断裂的过程。在早期种子发育过程中由 Gc 因子诱导，在后期阶段受到 DNA 甲基化的抑制。
The cuckoo chromosome 4S(L) from Aegilops sharonensis is preferentially transmitted when introduced by hybridization into common wheat, Triticum aestivum, Gametocidal (Gc) factors carried in 4S(L) induce chromosome breakage in meiospores not containing them, ensuring their transmission to the progeny. Chromosome breakage and break-fusion-bridge (BFB) cycles can also be observed during early embryo sac development of chromosome 4S(L) addition lines to wheat. often leading to the presence of dicentric chromosomes in the subsequent progeny. However. the process responsible for inducing the primary chromosomal breaks only appears to occur during the initial divisions of the embryo and endosperm. In the presence of chromosome 4S(L). treatment with the hypomethylating agent 5-azacytidine induces chromosome breakage in root tips. This suggests that the process of chromosome fragmentation. induced by the Gc factors during early seed development, is repressed at later stages by DNA methylation.