PRIOR EXERCISE SUPPRESSES THE PLASMA TUMOR-NECROSIS-FACTOR RESPONSE TO BACTERIAL LIPOPOLYSACCHARIDE
PRIOR EXERCISE SUPPRESSES THE PLASMA TUMOR-NECROSIS-FACTOR RESPONSE TO BACTERIAL LIPOPOLYSACCHARIDE
复制标题
DOI：
10.1152/jappl.1994.77.3.1542
复制
发表时间：
1994-09-01
影响因子：
3.3
通讯作者：
SHEPHERD, RE
中科院分区：
文献类型：
--
作者：
BAGBY, GJ;SAWAYA, DE;SHEPHERD, RE
本研究旨在确定体育锻炼对体内肿瘤坏死因子-α (TNF) 对大肠杆菌脂多糖 (LPS) 反应的影响。熟悉跑步机跑步并通过手术植入血管导管的大鼠在静脉注射 LPS 攻击（1 mg/kg；24 小时内剂量致死率为 10-20%）之前不运动或运动至接近精疲力尽（平均跑步时间为 102 +/- 13 分钟）。与时间匹配的未运动对照大鼠相比，运动大鼠的心率、血浆乳酸和血浆皮质酮增加，运动结束时血糖降低。由于 LPS，两组均出现低血压，表现出短暂的高血糖和持续的高乳酸血症。 LPS 后 30 分钟，两组血浆皮质酮水平相似。未运动的大鼠对 LPS 表现出正常的血浆 TNF 反应，峰值 (10,400 +/- 2,000 U/ml) 出现在 LPS 攻击后 90 分钟。相比之下，LPS 给药前运动的大鼠的 TNF 反应减弱为未运动组的 17%，峰值出现在 LPS 后较早的时间。将重组鼠TNF添加到运动后血浆中得到完全表达。当运动完成后 6 小时内给予 LPS 时，TNF 反应仍然减弱，但在恢复 24 小时的大鼠中，TNF 反应恢复正常。结果表明，运动可能作为一种压力方式，显着抑制通常在应对 LPS 挑战时观察到的全身 TNF 反应。
This study was initiated to determine the effect of physical exercise on the in vivo tumor necrosis factor-alpha (TNF) response to Escherichia coli lipopolysaccharide (LPS). Rats familiarized with treadmill running and surgically implanted with vascular catheters were either not exercised or exercised to near exhaustion (mean run time of 102 +/- 13 min) before intravenous LPS challenge (1 mg/kg; lethality of dose is 10-20% in 24 h). Compared with time-matched nonexercised control rats, exercised rats had increased heart rates, plasma lactate, and plasma corticosterone and decreased plasma glucose at the conclusion of exercise. In response to LPS, both groups became hypotensive, exhibited transient hyperglycemia, and sustained hyperlactacidemia. By 30 min post-LPS, plasma corticosterone levels were similar in the two groups. Nonexercised rats exhibited a normal plasma TNF response to LPS with the peak value (10,400 +/- 2,000 U/ml) occurring 90 min after LPS challenge. In contrast, the TNF response in rats exercised before LPS administration was blunted to 17% of the nonexercised group, with the peak occurring at an earlier time after LPS. Addition of recombinant murine TNF to postexercise plasma was fully expressed. The TNF response remained attenuated when LPS was administered up to 6 h after completion of exercise, but it returned to normal in rats allowed to recover for 24 h. The results demonstrate that exercise, perhaps as a stress modality, markedly suppresses the systemic TNF response that is normally observed in response to LPS challenge.