Force generated by human sperm correlated to velocity and determined using a laser generated optical trap.
Force generated by human sperm correlated to velocity and determined using a laser generated optical trap.
复制标题
人类精子产生的力与速度相关，并使用激光产生的光陷阱确定。
DOI：
10.1016/s0015-0282(16)53539-0
复制
发表时间：
1990
影响因子：
6.7
通讯作者：
Berns,MW
中科院分区：
文献类型：
--
作者：
Tadir,Y;Wright,WH;Vafa,O;Ord,T;Asch,RH;Berns,MW
单束梯度力光阱的发展使得仅通过激光操纵细胞成为可能。将连续波 Nd:YAG（1.06 微米）激光束导入传统显微镜并通过物镜聚焦到观察平面上。测量了人类精子从捕获器中释放时的激光束功率，并将其与捕获前精子的线速度相关联。慢速、中速和快速运动精子的平均捕获功率读数分别为 57、73 和 84 mW。对总人口测量值的分析表明，与具有相似平均线速度的直线运动精子相比，之字形运动精子具有显着更高的平均功率读数。在两种情况下，24 小时后重复测量时，样本所需的捕获能力显着降低。
The development of the single beam gradient force optical trap has made it possible to manipulate cells solely by laser light. A continuous wave Nd:YAG (1.06 microns) laser beam was directed into a conventional microscope and focused onto the viewing plane by the objective lens. The laser beam power at which human sperm were released from the trap was measured and correlated to the sperm's linear velocity before trapping. The mean trapping power readings for slow, medium, and fast motile sperm were 57, 73, and 84 mW, respectively. The analysis of measurements over the total population demonstrated that zig-zag motile sperm had significantly higher mean power readings when compared with straight motile sperm with similar mean linear velocities. In two cases, specimens required significantly less trapping power when the measurements were repeated 24 hours later.