Sendai virus vector-mediated transgene expression in the cochlea in vivo
Sendai virus vector-mediated transgene expression in the cochlea in vivo
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DOI：
10.1159/000097798
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发表时间：
2007-01-01
影响因子：
1.6
通讯作者：
Ogawa, Kaoru
中科院分区：
文献类型：
--
作者：
Kanzaki, Sho;Shiotani, Akihiro;Ogawa, Kaoru
我们使用两种不同的方法（介质阶和鼓阶）将重组仙台病毒 (SeV) 载体注射到豚鼠耳蜗中，并研究了哪些细胞类型吸收了该载体。与使用鼓阶方法的动物相比，使用介质阶方法的动物的听阈偏移和转染细胞的分布是不同的。 SeV可以转染非常不同类型的细胞，包括血管纹、螺旋神经节神经元、柯蒂氏器的感觉上皮以及鼓阶的纤维细胞。由于 SeV 载体可以向耳蜗传递刺激以诱导毛细胞再生，因此它可能是修复柯蒂氏器的有力工具。版权所有 (C) 2007 S. Karger AG，巴塞尔。
We injected a recombinant Sendai virus ( SeV) vector into the guinea pig cochlea using two different approaches - the scala media and scala tympani - and investigated which cell types took up the vector. The hearing threshold shift and distribution of transfected cells in animals using the scala media approach were different compared to those using the scala tympani approach. SeV can transfect very different types of cells, including stria vascularis, spiral ganglion neurons, and sensory epithelia of the organ of Corti, and fibrocytes of the scala tympani. Because SeV vectors can potentially deliver stimuli to the cochlea to induce hair cell regeneration, it may be a powerful tool for repairing the organ of Corti. Copyright (C) 2007 S. Karger AG, Basel.