Artificial "ping-pong" cascade of PIWI-interacting RNA in silkworm cells
Artificial "ping-pong" cascade of PIWI-interacting RNA in silkworm cells
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家蚕细胞中 PIWI 相互作用 RNA 的人工“乒乓”级联
作者：
Shoji K;Suzuki Y;Sugano S;Shimada T;Katsuma S.
蚕雄性化（Masc）基因编码一个CCCH串联锌指蛋白，该蛋白控制雄性化和剂量补偿。Masc蛋白是一种核蛋白，但其转运进入细胞核的机制尚未阐明。在这里，我们发现了一个位于Masc蛋白残基274和290之间的功能性二部核定位信号（NLS）。序列比较表明，该双侧NLS在其他鳞翅目昆虫的Masc蛋白中具有进化保守性。此外，我们发现核定位的程度与Masc蛋白的雄性化活性无关。
The silkwormMasculinizer(Masc) gene encodes a CCCH‐tandem zinc finger protein that controls both masculinization and dosage compensation. Masc protein is a nuclear protein, but the mechanism underlying the transport of this protein into the nucleus has not yet been elucidated. Here, we identified a functional bipartite nuclear localization signal (NLS) located between residues 274 and 290 of the Masc protein. Sequence comparison revealed that this bipartite NLS is evolutionarily conserved in Masc proteins from other lepidopteran insects. Furthermore, we showed that the degree of nuclear localization is not associated with the masculinizing activity of the Masc protein.