Expression of genes encoding mineralocorticoid biosynthetic enzymes and the mineralocorticoid receptor, and levels of mineralocorticoids in the bovine follicle and corpus luteum
Expression of genes encoding mineralocorticoid biosynthetic enzymes and the mineralocorticoid receptor, and levels of mineralocorticoids in the bovine follicle and corpus luteum
复制标题
DOI：
10.1262/jrd.2019-127
复制
发表时间：
2020-02-01
影响因子：
1.8
通讯作者：
Tetsuka, Masafumi
中科院分区：
文献类型：
--
作者：
Mukangwa, Memory;Takizawa, Koki;Tetsuka, Masafumi
与性类固醇不同，盐皮质激素在卵巢生理学中引起的关注有限。最近对灵长类动物的研究表明，盐皮质激素可能在卵巢局部合成和发挥作用。在这里，我们研究了牛卵巢中盐皮质激素水平和编码其生物合成酶和受体的基因表达的发育变化。从F1小母牛中采集卵泡和黄体（CL）。21 α-羟化酶（CYP 21 A2），11 β-羟化酶-1（CYP 11B 1）和盐皮质激素受体（NR 3C 2）在颗粒细胞（GC），卵泡膜层（TL）和CL组织中的表达水平通过实时PCR进行定量，而卵泡液中的盐皮质激素通过酶免疫测定（EIA）进行测量。TL和GC表达CYP 21 A2和NR 3C 2，而CYP 11B 1以非常低或不可检测的水平表达。这些基因的表达水平在小/大和健康/闭锁卵泡之间没有显着差异，但在TL中高于GC。CYP 21 A2和NR 3C 2在所有CL阶段均有表达，中期表达较高。CYP 11B 1表达仅在中期CL中明显。在所有卵泡中均检测到醛固酮，且其浓度在卵泡组之间无显著差异。在成对的大健康/闭锁卵泡中，脱氧皮质酮（醛固酮的前体）的浓度约为醛固酮的10倍，健康卵泡和闭锁卵泡之间无显著差异。总之，牛卵泡中盐皮质激素和NR 3C 2的表达以及CL中CYP 21 A2、CYP 11B 1和NR 3C 2表达的发育变化表明盐皮质激素在牛卵巢中可能具有内分泌/旁分泌/自分泌作用。
Unlike sex steroids, mineralocorticoids have attracted limited attention in ovarian physiology. Recent studies on primates have indicated possible local synthesis and action of mineralocorticoids in the ovary. Here, we examined developmental changes in the levels of mineralocorticoids and expression of genes encoding their biosynthetic enzymes and receptor in the bovine ovary. The follicles and corpora lutea (CL) were collected from F1 heifers. Expression levels of 21 alpha-hydroxylase (CYP21A2), 11 beta-hydroxylase-1 (CYP11B1), and the mineralocorticoid receptor (NR3C2) in granulosa cells (GC), thecal layers (TL), and CL tissues were quantified by real-time PCR, whereas mineralocorticoids in the follicular fluid were measured by enzyme immunoassay (EIA). TL and GC expressed CYP21A2 and NR3C2, whereas CYP11B1 was expressed at very low or undetectable levels. The expression levels of these genes were not significantly different among small/large and healthy/atretic follicles but were higher in TL than in GC. CYP21A2 and NR3C2 were expressed in all CL stages with higher expression observed in the mid-stage. CYP11B1 expression was only apparent in the mid-stage CL. Aldosterone was detected in all follicles, and its concentration was not significantly different among the follicular groups. In paired large-healthy/atretic follicles, the concentration of deoxycorticosterone, a precursor of aldosterone, was approximately ten-fold higher than that of aldosterone and not significantly different between healthy and atretic follicles. In conclusion, the presence of mineralocorticoids and expression of NR3C2 in the bovine follicle together with the developmental change in the expression of CYP21A2, CYP11B1, and NR3C2 in the CL suggest possible endocrine/paracrine/autocrine roles of mineralocorticoids in the bovine ovary.