Foveolar differentiation of mouse gastric mucosa in vitro
Foveolar differentiation of mouse gastric mucosa in vitro
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DOI：
10.1016/s0002-9440(10)64324-6
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发表时间：
2003-06-01
影响因子：
6
通讯作者：
Sugihara, H
中科院分区：
文献类型：
--
作者：
Ootani, A;Toda, S;Sugihara, H
我们报告了一种新的方法，可以培养高度分化的胃表面粘膜细胞。分离的小鼠胃上皮细胞和成纤维细胞在三维胶原凝胶体系中共培养，重建的粘膜表面用气液界面处理。对培养细胞进行组织学、免疫组织化学和电镜检查。分离的上皮细胞MUC5AC阳性，在无细胞胶原凝胶上显示未成熟的粘液细胞特征（坑前细胞期）。然而，当给予成纤维细胞支持时，上皮细胞分化为成熟的表面黏液细胞（坑细胞期），并显示出高柱状细胞形状，基底圆形细胞核和充满黏液的细胞质。精细结构上，细胞呈连接复合物、基底层、糖原和秘书颗粒。气液界面环境的进一步处理改变了坑细胞的分化状态（坑顶细胞阶段）；导致组织蛋白酶E的表达，糖原颗粒的消失，分泌颗粒的顶部堆积，凋亡细胞的增加。该培养模型将为研究胃上皮细胞生物学和胃粘膜各种疾病提供有用的工具。
We report a novel method that allows the culture of highly differentiated gastric surface mucous cells. isolated mouse gastric epithelial cells and fibroblasts were co-cultured in a three-dimensional collagen gel system, and the reconstructed mucosal surface treated with an air-liquid interface. Cultured cells were examined by histology, immunohistochemistry, and electron microscopy. Isolated epithelial cells were positive for MUC5AC, and showed immature mucous cell features (pre-pit cell stage) on cell-free collagen gel. However, when given fibroblastic support, the epithelial cells differentiated into mature surface mucous cells (pit cell stage), and showed a tall columnar cell shape, basal round nuclei, and mucus-filled cytoplasm. In the fine structure, the cells showed Junctional complexes, basal Lamina, and glycogen and secretary granules. Further treatment by the air-liquid interface environment modified the differentiated state of the pit cells (pit top cell stage); resulting in the expression of cathepsin E, the disappearance of glycogen granules and the apical accumulation of secretory granules along with an increase in apoptotic cells. This culture model should provide a useful tool for studying gastric epithelial cell biology and various diseases of the gastric mucosa.