BIOCHEMICAL-CHANGES AND CYTOTOXICITY ASSOCIATED WITH THE DEGRADATION OF POLYMERIC GLUTARALDEHYDE DERIVED CROSS-LINKS
BIOCHEMICAL-CHANGES AND CYTOTOXICITY ASSOCIATED WITH THE DEGRADATION OF POLYMERIC GLUTARALDEHYDE DERIVED CROSS-LINKS
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DOI：
10.1002/jbm.820240905
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发表时间：
1990-09-01
期刊：
影响因子：
--
通讯作者：
NIMNI, ME
中科院分区：
文献类型：
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作者：
HUANGLEE, LLH;CHEUNG, DT;NIMNI, ME
戊二醛交联的可逆性被认为是长期生物假体植入物失效的原因。在肌腱和模型化合物中研究了这种交联的稳定性。即使在这些肌腱被广泛冲洗长达6个月后，交联肌腱仍释放出少量但具有细胞毒性的戊二醛。通过冲洗的交联肌腱中段周围成纤维细胞的死亡证明了毒性作用，而末端部分未显示毒性作用。研究了戊二醛修饰的[14 C]-L-赖氨酸衍生物的形成和稳定性。戊二醛与氨基化合物的聚合反应最初很快，但在几个月内继续缓慢进行。通过凝胶过滤色谱法检测高分子量可溶性聚合物的降解。低分子量的可溶性物质也从不溶性产物中释放出来，这些不溶性产物是在高浓度的戊二醛和放射性赖氨酸反应时形成的。这些化学和生物学研究表明，戊二醛交联生物假体的局部细胞毒性可能是由于交联组织间隙中存在不稳定的戊二醛聚合物。
The reversibility of glutaraldehyde crosslinks has been suggested as a reason for failure of long-term bioprosthetic implants. The stability of such crosslinks was investigated in tendons and model compounds. Small but cytotoxic levels of glutaraldehyde were still released from crosslinked tendons even after these tendons were extensively rinsed for up to 6 months. The toxic effect was evidenced by the death of fibroblasts surrounding a midsection piece of rinsed crosslinked tendon, while the end section pieces did not show toxic effects. The formation and stability of glutaraldehyde modified [14C]-L-lysine derivatives were investigated. The polymerization of glutarldehyde with amino compounds was initially fast but continued to proceed slowly for months. Degradation of high-molecular-weight-soluble polymers was detected by gel filtration chromatography. Low-molecular-weight soluble materials were also released from insoluble products which were formed when high concentrations of glutaraldehyde and radioactive lysine were reacted. These chemical and biological studies suggest that local cytotoxicity of glutaraldehyde crosslinked bioprotheses may be due to unstable glutaraldehyde polymers that persist in the interstices of crosslinked tissues.