Expression of fibroblast growth factors in rat dorsal root ganglion neurons and regulation after peripheral nerve injury
Expression of fibroblast growth factors in rat dorsal root ganglion neurons and regulation after peripheral nerve injury
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DOI：
10.1097/00001756-200210280-00014
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发表时间：
2002-10-28
期刊：
影响因子：
1.7
通讯作者：
Zhang, X
中科院分区：
文献类型：
--
作者：
Li, GD;Wo, Y;Zhang, X
利用cDNA阵列技术，我们观察了成纤维细胞生长因子（FGF）家族8个成员FGF 2、5、7、9、10、13和14在大鼠腰4和5背根神经节（DRGs）中的表达。坐骨神经横断后28天，drg中FGF 2和FGF 7的阵列信号明显增加，FGF 13的阵列信号明显减少。利用逆转录聚合酶链反应（RT-PCR），我们证实了肛肠切开术诱导的FGF 7和13的表达变化。原位杂交结果显示，正常情况下60%的DRG神经元表达FGF 13。术后7 d FGF - 13阳性神经元数量下降至18%，28 d部分恢复至40%。FGF - 13的免疫反应性也下降。这些数据表明，在正常情况下和神经损伤后，FGFs对DRG神经元很重要。
Using cDNA array, we observed the expression of eight members of the fibroblast growth factor (FGF) family, FGF 2, 5, 7,9,10,13 and 14, in rat lumbar 4 and 5 dorsal root ganglia (DRGs). Over a period of 28 days after sciatic nerve transection, the array signals for FGF 2 and 7 were significantly increased in the DRGs, while FGF 13 decreased. Using the reverse transcription polymerase chain reaction (RT-PCR), we confirmed the axotomy-induced changes in the expression of FGF 7 and 13. In situ hybridization showed that FGF 13 was expressed in 60% of DRG neurons under normal circumstance. Seven days after axotomy the number of FGF 13-positive neurons was decreased to 18%, but partially recovered to 40% after 28 days. FGF 13 immunoreactivity was also decreased. These data indicate that FGFs are important for DRG neurons under normal circumstance and after nerve injury.