Effect of iron-sulfur cluster assembly proteins on the expression of Escherichia coli lipoic acid synthase
Effect of iron-sulfur cluster assembly proteins on the expression of Escherichia coli lipoic acid synthase
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DOI：
10.1016/s1046-5928(02)00680-0
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发表时间：
2003-04-01
影响因子：
1.6
通讯作者：
Roach, PL
中科院分区：
文献类型：
--
作者：
Kriek, M;Peters, L;Roach, PL
硫辛酸合成酶（利帕）可以容纳[4Fe-4S]簇，该簇被认为是在硫辛酸的生物合成期间硫插入辛酰基底物中所必需的。为了改善可溶性全利帕表达，构建了一系列携带利帕与三种系统之一的多顺反子质粒：groE/SL、trxA或isc操纵子。利帕A与isc操纵子的共表达使可溶性利帕A的分离产量大约增加了两倍，并导致Fe-S簇的有效组装。这种策略可能有助于可溶性表达广泛的Fe-S簇依赖性蛋白。(C)2002年爱思唯尔科学（美国）。All rights reserved.
Lipoic Acid Synthase (LipA) can accommodate a [4Fe-4S] cluster that is thought to be essential for the insertion of sulfur into an octanoyl substrate during the biosynthesis of lipoic acid. With the objective of improving soluble holo-LipA expression, a series of multi-cistronic plasmids were constructed carrying lipA in combination with one of the three systems: groE/SL, trxA, or the isc operon. Co-expression of lipA with the isc operon approximately trebled the isolated yield of soluble LipA and resulted in efficient assembly of the Fe-S cluster. This strategy may be helpful in the soluble expression of a wide range of Fe-S cluster-dependent proteins. (C) 2002 Elsevier Science (USA). All rights reserved.