TRITON X-100 ALTERS THE RESISTANCE LEVEL OF METHICILLIN-RESISTANT STAPHYLOCOCCUS-AUREUS TO OXACILLIN
TRITON X-100 ALTERS THE RESISTANCE LEVEL OF METHICILLIN-RESISTANT STAPHYLOCOCCUS-AUREUS TO OXACILLIN
复制标题
DOI：
10.1111/j.1574-6968.1995.tb07939.x
复制
发表时间：
1995-12-15
影响因子：
2.1
通讯作者：
SUGINAKA, H
中科院分区：
文献类型：
--
作者：
KOMATSUZAWA, H;SUGAI, M;SUGINAKA, H
我们用群体分析的方法检测了Triton X-100对18株耐甲氧西林金黄色葡萄球菌对苯唑西林耐药性的影响。在0.02%Triton X-100存在下，17株原来对甲氧西林耐药的菌株对苯唑西林表现出更高的敏感性。同质分离株KSAF1几乎不受Triton X-100的影响。当细菌生长在0.02%的Triton X-100中时，溶葡萄球菌酶、51 kDa N-乙酰氨基葡萄糖苷酶和62 kDa N-乙酰壁氨酰-L-丙氨酸酰胺酶对热在激活细胞的敏感性不受影响。我们的数据和以前的研究表明，Triton X-100通过影响除PBPs、溶菌酶或FemAB产物以外的一个因素(S)来改变耐甲氧西林金黄色葡萄球菌的耐药性水平。
We used population analysis to examine the effects of Triton X-100 on the level of resistance to oxacillin of 18 methicillin-resistant Staphylococcus aureus. In the presence of 0.02% Triton X-100, 17 formerly methicillin-resistant strains exhibited enhanced sensitivity to oxacillin. One homogeneous isolate, KSAF1 was barely affected by the Triton X-100. Sensitivities of lysostaphin, 51 kDa N-acetylglucosaminidase and 62 kDa N-acetylmuramoyl-L-alanine amidase to heat-in-activated cells were not affected when the bacteria were grown in 0.02% Triton X-100. Our data, together with those of a previous study, suggested that Triton X-100 alters the resistance level of methicillin-resistant S. aureus by influencing a factor(s) other than PBPs, bacteriolytic enzymes, or femAB products.