Regional differences in the delayed rectifier current (IKr and IKs) contribute to the differences in action potential duration in basal left ventricular myocytes in guinea-pig
Regional differences in the delayed rectifier current (IKr and IKs) contribute to the differences in action potential duration in basal left ventricular myocytes in guinea-pig
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DOI：
10.1016/s0008-6363(98)00133-3
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发表时间：
1998-11-01
影响因子：
10.8
通讯作者：
Hart, G
中科院分区：
文献类型：
--
作者：
Bryant, SM;Wan, XP;Hart, G
目的：比较从左心室基底区不同层分离的单个心肌细胞的特性，并检验延迟整流电流（I-k）的差异导致动作电位时程区域差异的假设。方法：分离豚鼠左心室基底膜下、中层和心外膜下心肌细胞。使用开关钳技术测量膜电压和电流。结果如下：在90%复极化时测量的平均动作电位时程（APD（90）），内膜下心肌细胞比中层心肌和心外膜下心肌细胞长[0.2 Hz时APD（90）ms：内膜下292 +/- 12（n = 40），中层心肌243 +/- 8（n = 42），心外膜下227 +/- 9（n = 36），P
Objective: To compare the properties of single myocytes isolated from different layers of the basal region of the left ventricle and to test the hypothesis that differences in the delayed rectifier current (I-k) contribute to regional differences in action potential duration. Methods: Myocytes were isolated from basal sub-endocardial, mid-myocardial and sub-epicardial layers of the guinea-pig left ventricle. Membrane voltage and current were measured using the switch-clamp technique. Results: Mean action potential duration measured at 90% repolarisation (APD(90)) was longer in sub-endocardial myocytes than in mid-myocardial and sub-epicardial myocytes [APD(90) ms at 0.2 Hz: sub-endocardial 292 +/- 12 (n = 40), mid-myocardial 243 +/- 8 (n = 42) and sub-epicardial 227 +/- 9 (n = 36), P