Inhibition of Pseudogymnoascus destructans Growth from Conidia and Mycelial Extension by Bacterially Produced Volatile Organic Compounds
Inhibition of Pseudogymnoascus destructans Growth from Conidia and Mycelial Extension by Bacterially Produced Volatile Organic Compounds
复制标题
DOI：
10.1007/s11046-013-9716-2
复制
发表时间：
2014-02-01
期刊：
影响因子：
5.5
通讯作者：
Crow, Sidney A., Jr.
中科院分区：
文献类型：
--
作者：
Cornelison, Christopher T.;Gabriel, Kyle T.;Crow, Sidney A., Jr.
自2006年首次记录以来，最近发现的白鼻综合征（WNS）病原体Pseudogymnoascus destructans与估计550万只北美蝙蝠的死亡有关（Frick et al. Science 329:679-682, 2010）。为了确定WNS的潜在生物和化学控制选择，筛选了6种先前描述的细菌产生的挥发性有机化合物（VOCs）的抗p。destructans活动。所述化合物包括癸醛；2-ethyl-1-hexanol;壬醛;苯并噻唑;苯甲醛;啊,N-dimethyloctylamine。在15℃和4℃的密闭空气环境中，对破坏性假单胞菌的分生孢子和菌丝塞进行暴露，评价其对生长的抑制作用。所有的挥发性有机化合物都能抑制分生孢子的生长，并抑制菌丝的径向延伸，在4℃时效果最大。对抑菌土壤生态学的研究和这些环境中存在的抑菌性挥发性有机化合物的自然丰度表明，某些挥发性有机化合物可能会产生协同作用。对两种或三种浓度相等的挥发性有机化合物配方的评价在若干情况下支持协同作用。细菌产生的挥发性有机化合物的抗p鉴定。destrucans的活性表明，抑制疾病和抑菌土壤是WNS生物和化学控制选择的潜在重要储存库，并为野生动物管理人员提供了与这种毁灭性疾病作斗争的工具。
The recently identified causative agent of white-nose syndrome (WNS), Pseudogymnoascus destructans, has been implicated in the mortality of an estimated 5.5 million North American bats since its initial documentation in 2006 (Frick et al. in Science 329:679-682, 2010). In an effort to identify potential biological and chemical control options for WNS, 6 previously described bacterially produced volatile organic compounds (VOCs) were screened for anti-P. destructans activity. The compounds include decanal; 2-ethyl-1-hexanol; nonanal; benzothiazole; benzaldehyde; andN,N-dimethyloctylamine. P. destructans conidia and mycelial plugs were exposed to the VOCs in a closed air space at 15 and 4 A degrees C and then evaluated for growth inhibition. All VOCs inhibited growth from conidia as well as inhibiting radial mycelial extension, with the greatest effect at 4 A degrees C. Studies of the ecology of fungistatic soils and the natural abundance of the fungistatic VOCs present in these environments suggest a synergistic activity of select VOCs may occur. The evaluation of formulations of two or three VOCs at equivalent concentrations was supportive of synergistic activity in several cases. The identification of bacterially produced VOCs with anti-P. destructans activity indicates disease-suppressive and fungistatic soils as a potentially significant reservoir of biological and chemical control options for WNS and provides wildlife management personnel with tools to combat this devastating disease.