Collagen (NeuraGen®) nerve conduits and stem cells for peripheral nerve gap repair
Collagen (NeuraGen®) nerve conduits and stem cells for peripheral nerve gap repair
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DOI：
10.1016/j.neulet.2014.04.029
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发表时间：
2014-06-20
影响因子：
2.5
通讯作者：
Kalbermatten, Daniel F.
中科院分区：
文献类型：
--
作者：
di Summa, Pietro G.;Kingham, Paul J.;Kalbermatten, Daniel F.
胶原蛋白神经导片在临床上用于周围神经缺损，但其使用通常仅限于病变3厘米。在这项研究中，我们将胶原导管与细胞结合，作为支持长间隙神经再生的替代策略。通过扫描电镜评估体外细胞对胶原导管（NeuraGen (R)神经导管）的粘附性。在体内实验中，将雪旺细胞（SC）、SC样分化骨髓间充质干细胞（dMSC）、SC样分化脂肪源性干细胞（dASC）或空管（对照组）植入导管，用导管在大鼠坐骨神经中架起1厘米的缺口，2周后进行免疫组化分析，评估轴突再生和SC浸润情况。再生细胞表现出良好的粘附性。初级SC在远端树桩发芽方面有显著改善。各组间近端再生距离无显著差异。加载dMSC和dasc的导管呈弥漫性发芽模式，而加载sc的导管呈增强的锥形模式，并沿导管壁典型地发芽，表明其对I型胶原纤维结构的亲和力增加。NeuraGen导引剂对再生细胞具有较高的亲和力，可作为细胞递送的有效载体。然而，NeuraGen指南的表面修饰（例如使用细胞外基质分子肽）可用于未来的组织工程应用，以更好地利用细胞潜力。2014爱思唯尔爱尔兰有限公司版权所有。
Collagen nerve guides are used clinically for peripheral nerve defects, but their use is generally limited to lesions up to 3 cm. In this study we combined collagen conduits with cells as an alternative strategy to support nerve regeneration over longer gaps. In vitro cell adherence to collagen conduits (NeuraGen (R) nerve guides) was assessed by scanning electron microscopy. For in vivo experiments, conduits were seeded with either Schwann cells (SC), SC-like differentiated bone marrow-derived mesenchymal stem cells (dMSC), SC-like differentiated adipose-derived stem cells (dASC) or left empty (control group), conduits were used to bridge a 1 cm gap in the rat sciatic nerve and after 2-weeks immunohistochemical analysis was performed to assess axonal regeneration and SC infiltration. The regenerative cells showed good adherence to the collagen walls. Primary SC showed significant improvement in distal stump sprouting. No significant differences in proximal regeneration distances were noticed among experimental groups. dMSC and dASC-loaded conduits showed a diffuse sprouting pattern, while SC-loaded showed an enhanced cone pattern and a typical sprouting along the conduits walls, suggesting an increased affinity for the collagen type I fibrillar structure. NeuraGen guides showed high affinity of regenerative cells and could be used as efficient vehicle for cell delivery. However, surface modifications (e.g. with extracellular matrix molecule peptides) of NeuraGen guides could be used in future tissue-engineering applications to better exploit the cell potential. (C) 2014 Elsevier Ireland Ltd. All rights reserved.