Developmental expression of vascular endothelial growth factor and its receptors in ovine placenta and fetal membranes
Developmental expression of vascular endothelial growth factor and its receptors in ovine placenta and fetal membranes
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DOI：
10.1016/s1071-5576(99)00016-7
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发表时间：
1999-07-01
期刊：
影响因子：
--
通讯作者：
Brace, RA
中科院分区：
文献类型：
--
作者：
Cheung, CY;Brace, RA
目的：人类胎盘表面以及包括绵羊在内的几种物种胎盘和胎膜表面的血管分布是羊水膜内吸收的重要决定因素。我们以前的研究表明，绵羊羊膜和绒毛膜中的总血管表面积随着妊娠的进行而增加。血管内皮生长因子（VEGF）是一种强有力的血管生成和渗透性因子，并发现在羊胎盘和胎膜中表达。为了探讨VEGF在维持膜内微血管吸收连接中的作用，本研究测定了妊娠期绵羊胎盘、绒毛膜和羊膜中VEGF及其受体、含激酶插入结构域受体（KDR）和fms样酪氨酸激酶（Flt-1）基因表达的变化。从妊娠60 - 140天的绵羊胎儿的胎盘子叶、绒毛膜和羊膜中提取总RNA。通过北方印迹分析确定VEGF、KDR和Flt-1 mRNA的相对丰度，并通过逆转录聚合酶链反应鉴定表达的VEGF分子形式。妊娠期;结果：在绵羊胎盘、绒毛膜和羊膜中，VEGFmRNA的表达量在60~140 d显著增加，而在绒毛膜和羊膜中，VEGFmRNA的表达量在60~140 d显著增加。在这些组织中表达的主要VEGF分子形式是VEGF（164），而VEGF（120）、VEGF（144）和VEGF（188）以较低水平存在。在胎盘中，KDR是表达的主要VEGF受体，尽管Flt-1 tvas也以非常低的水平检测。在羊膜和绒毛膜中，KDR作为唯一的受体表达。在胎盘和membranes.CONCLUSIONS：VEGF基因表达的增加与妊娠推进在羊膜和绒毛膜中，KDR的表达表明，VEGF及其受体的血管和渗透性的重要决定因素，从而交换能力，膜内途径。版权所有（C）1999年由妇科调查协会。
OBJECTIVES: Vascularity of the surface of the placenta in humans and of the placenta and fetal membranes in several species including sheep is an important determinant of intramembranous absorption of amniotic fluid. Our previous studies have shown that the total blood vessel surface area in ovine amnion and chorion increases with advancing gestation. Vascular endothelial growth factor (VEGF) is a potent angiogenic and permeability factor and is found to be expressed in the ovine placenta and fetal membranes. To investigate the role of VEGF in maintaining the absorptive junction of the intramembranous microvessels, the present study was undertaken to determine the gestational change in gene expression of VEGF and its receptors, kinase insert domain-containing receptor (KDR) and fms-like tyrosine kinase (Flt-1), in ovine placenta, chorion, and amnion.METHODS: Total RNA was extracted from placental cotyledon, chorion, and amnion of ovine fetuses at 60-140 days of gestation. The relative abundance of VEGF, KDR, and Flt-1 mRNA was determined by Northern blot analysis, and VEGF molecular forms expressed were identified by reverse transcriptase polymerase chain reaction. The gestational; changes in mRNA levels of VEGF and its receptors were analyzed by regression analysis.RESULTS: In ovine placenta, chorion, and amnion, VEGF mRNA levels increases significantly from 60 to 140 days. The major VEGF molecular form expressed in these tissues was VEGF(164), whereas VEGF(120), VEGF(144), and VEGF(188) were present at lower levels, In the placenta, KDR was the primary VEGF receptor expressed, although Flt-1 tvas also detected at very low levels. In the amnion and chorion, KDR li,as the only receptor expressed. A gestational-dependent change in VEGF receptor expression was not observed in the placenta and membranes.CONCLUSIONS: The increase in VEGF gene expression with advancing gestation in the amnion and chorion where KDR is expressed suggests that VEGF and its receptor are important determinants of vascularity and permeability, and thus exchange capacity, of the intramembranous pathway. Copyright (C) 1999 by the Society for Gynecologic Investigation.