Micropatterning of HeLa cells on glass substrates and evaluation of respiratory activity using microelectrodes
Micropatterning of HeLa cells on glass substrates and evaluation of respiratory activity using microelectrodes
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DOI：
10.1021/la011576k
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发表时间：
2002-04-30
期刊：
影响因子：
3.9
通讯作者：
Matsue, T
中科院分区：
文献类型：
--
作者：
Nishizawa, M;Takoh, K;Matsue, T
在玻璃基板上制备哺乳动物细胞（HeLa 细胞）的微图案，并通过微电极技术（主要是扫描电化学显微镜（SECM））研究图案化细胞的呼吸。通过将细胞外基质蛋白纤连蛋白微接触印刷到疏水性玻璃板上来制备微米级的细胞图案。通过扫描 Pt 微电极绘制图案化细胞附近的氧气浓度，获得的 SECM 图像证明图案化的细胞在吸收氧气的情况下存活。带状图案中的 HeLa 细胞分布良好，而小岛状图案中的细胞形状受到限制。通过测量本体溶液和细胞表面之间的氧浓度差异来评估这些细胞的呼吸活动，结果表明，扩散的细胞比圆形细胞消耗更多的氧气。
The micropatterns of mammalian cells (HeLa cells) were prepared on glass substrates, and the respiration of the patterned cells was studied by microelectrode techniques, mainly by scanning electrochemical microscopy (SECM). The cellular patterns on a micrometer scale were prepared by microcontact printing of an extracellular matrix protein, fibronectin, onto a hydrophobic glass plate. The oxygen concentration in the vicinity of the patterned cells was mapped by scanning a Pt microelectrode, and the obtained SECM images proved that the cells in patterns were living with the uptake of oxygen. HeLa cells in the band patterns were well spread, while the cells in the small island patterns were restricted in their shape. The respiratory activities of these cells were evaluated by measuring the difference in the oxygen concentration between the bulk solution and the cell surface, and it was shown that a spreading cell consumed a significantly larger amount of oxygen than a round cell.