Survival, regeneration and functional recovery of motoneurons in adult rats by reirnplantation of ventral root following spinal root avulsion
Survival, regeneration and functional recovery of motoneurons in adult rats by reirnplantation of ventral root following spinal root avulsion
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DOI：
10.1111/j.0953-816x.2004.03295.x
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发表时间：
2004-04-01
影响因子：
3.4
通讯作者：
Wu, WT
中科院分区：
文献类型：
--
作者：
Gu, HY;Chai, H;Wu, WT
我们研究了在牵拉损伤的大鼠模型中，将撕脱的前根再植后运动神经元的功能恢复。通过控制性牵拉将第八颈根（C8）撕脱并立即再植到脊髓。结扎并切断相邻节段（C5、C6、C7和T1）的脊神经。12周或20周后，通过尼氏染色、运动神经元逆行标记、NOS组织化学、肌肉和神经-肌肉接头组织学检查、肌电图和行为学观察来评价脊髓运动神经元的存活、再生和功能恢复。在对照组动物中，分别有14%和11%的脊髓运动神经元在损伤后12周和20周存活。相比之下，在前根再植的动物中，分别有62%和55%的运动神经元在损伤后12周和20周存活。逆行标记和组织学检查表明，大约90%的存活的运动神经元在C8段再生轴突到回植的前根。用银和胆碱酯酶染色的肌肉揭示了新的运动终板的神经支配的肌肉。实验动物的指浅屈肌和桡侧腕屈肌均出现功能显著的肌电反应，但运动动作电位的平均潜伏期较正常对照组长。抓握试验显示手指屈肌和正中神经功能恢复。总之，我们的研究结果表明，脊髓运动神经元可以再生轴突通过再植根和再神经支配肌肉恢复部分功能。
We investigated the functional recovery of motoneurons after reimplanting an avulsed ventral root in a rat model of traction injury The eighth cervical root (C8) was avulsed by controlled traction and immediately reimplanted to the spinal cord. Spinal nerves from neighbouring segments (C5, C6, C7 and T1) were ligated and cut. After 12 or 20 weeks, the survival, regeneration and functional recovery of spinal motoneurons were evaluated by Nissl staining, retrograde labelling of motoneurons, NOS histochemistry, histological examination of muscle and nerve-muscle junction, electromyography and behavioural observation. In the control animals, about 14% or 11% of spinal motoneurons survived 12 or 20 weeks postinjury, respectively. By contrast, in animals with ventral root reimplantation, 62% and 55% of motoneurons survived at 12 or 20 weeks postinjury, respectively. Retrograde labelling and histological examination indicated that about 90% of the surviving motoneurons in the C8 segment regenerated axons into the reimplanted ventral root. Staining the muscles with silver and cholinesterase revealed new motor endplates in the reinnervated muscle. Functionally significant electromyographic responses in flexor digitorum superficialis and flexor carpi radialis were observed in experimental animals; however, the average latency of the motor action potentials was greater than normal control. The grasping test showed functional recovery of finger flexors and median nerve. In conclusion, our results indicate that spinal motoneurons can regenerate axons through reimplanted roots and reinnervate muscles to recover partial function.