Surface-enhanced Raman scattering analysis of urine from deceased donors as a prognostic tool for kidney transplant outcome.
Surface-enhanced Raman scattering analysis of urine from deceased donors as a prognostic tool for kidney transplant outcome.
复制标题
DOI：
10.1002/jbio.201700019
复制
发表时间：
2017-12
影响因子：
2.8
通讯作者：
Du H
中科院分区：
文献类型：
--
作者：
Chi J;Ma Y;Weng FL;Thiessen-Philbrook H;Parikh CR;Du H
我们报告了已故供者尿液表面增强拉曼散射（SERS）分析对肾移植预后的应用。碘化修饰银纳米颗粒被用作尿蛋白敏感测量的使能剂。采用主成分分析（PCA）和线性判别分析（LDA）对SERS数据进行统计分析。对三个班级的30份尿样进行了分析。ATN类别包括肾脏有急性肾小管坏死（ATN）的供体，这是最常见的急性肾损伤（AKI）类型，受者移植表现差的风险高，但移植结果可接受。DGF组由肾移植功能延迟（DGF）的供体组成。对照组包括肾脏没有供体ATN或受体DGF的供体。我们显示在区分ATN类与DGF和控制类方面的灵敏度超过90%。因此，我们的方法可以帮助临床医生选择高风险ATN类别的肾脏进行移植，否则将被丢弃。我们的研究是有影响力的，因为它可以作为一个有价值的指导，以扩大已故供体池，包括那些被认为是基于常见的尿液生物标志物的高风险AKI类型。已故供者尿液样本SERS分析用于肾移植结果指征的方案。
We report the utility of surface-enhanced Raman scattering (SERS) analysis of urine from deceased donors for prognosis of kidney transplant outcomes. Iodide-modified silver nanoparticles were used as the enabler for sensitive measurements of urine proteins. Principal component analysis (PCA) and linear discriminant analysis (LDA) were employed for the statistical analysis of the SERS data. Thirty urine samples in three classes were analysed. The ATN class consists of donors whose kidneys had acute tubular necrosis (ATN), the most common type of acute kidney injury (AKI) with high risk of poor graft performance in recipients, yet yielded acceptable transplant outcome. The DGF class is comprised of donors whose kidney had delayed graft function (DGF) in recipients. The control class includes donors whose kidneys did not have donor ATN or recipient DGF. We show a sensitivity of more than 90% in differentiating the ATN class from the DGF and control classes. Our methodology can thus help clinicians choose kidneys in the high-risk ATN category for transplant which would otherwise be discarded. Our research is impactful in that it could serve as a valuable guidance to expand the deceased donor pool to include those perceived as high-risk AKI type based on common urinary biomarkers. Scheme of SERS analysis of urine samples from deceased donors for kidney transplant outcome indication.
登录
查看更多内容
影响因子：
3.9
作者：
Kaaki, Karine;Herve-Aubert, Katel;Chourpa, Igor
通讯作者：
Chourpa, Igor
影响因子：
19.6
作者：
Hollmen, Maria E.;Kyllonen, Lauri E.;Salmela, Kaija T.
通讯作者：
Salmela, Kaija T.
DOI：
10.1097/01.asn.0000079042.13465.1a
发表时间：
2003-08-01
影响因子：
13.6
作者：
Mehta, RL;Chertow, GM
通讯作者：
Chertow, GM
影响因子：
8.8
作者：
Kayler, L. K.;Garzon, P.;Schold, J. D.
通讯作者：
Schold, J. D.
影响因子：
15
作者：
Hu, SZ;Smith, KM;Spiro, TG
通讯作者：
Spiro, TG