A High-Efficiency Method for the Production of Endothelial Cells from Human Induced Pluripotent Stem Cells.
A High-Efficiency Method for the Production of Endothelial Cells from Human Induced Pluripotent Stem Cells.
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DOI：
10.1007/7651_2021_377
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
Kogut I
中科院分区：
文献类型：
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作者：
Pavlova M;McGarvey SS;Bilousova G;Kogut I
内皮细胞是循环系统的重要组成部分。这些细胞可用于心血管疾病的体外建模和再生医学，以促进工程组织构建物的血管化。然而，低增殖能力和患者间的变异性限制了原代EC在临床和疾病建模中的使用。从人诱导多能干细胞（iPSC）分化的EC可以作为这些应用的原代EC的可行替代品。这是因为人类iPSC可以无限增殖，并有潜力分化成各种体细胞系，提供患者特异性细胞的可再生来源。在这里，我们提出了一种优化的，高度可重复的方法，用于人iPSC向血管EC分化。该方案依赖于WNT信号通路的激活以及生长因子和小分子的使用。所得到的iPSC衍生的EC可以培养多次传代而不丧失其功能，并且适合于体外和体内研究。
Endothelial cells (ECs) are important components of the circulatory system. These cells can be used for in vitro modeling of cardiovascular diseases and in regenerative medicine to promote vascularization of engineered tissue constructs. However, low proliferative capacity and patient-to-patient variability limit the use of primary ECs in the clinic and disease modeling. ECs differentiated from human induced pluripotent stem cells (iPSCs) can serve as a viable alternative to primary ECs for these applications. This is because human iPSCs can proliferate indefinitely and have the potential to differentiate into a variety of somatic cell lines, providing a renewable source of patient-specific cells. Here, we present an optimized, highly reproducible method for the differentiation of human iPSCs toward vascular ECs. The protocol relies on the activation of the WNT signaling pathway and the use of growth factors and small molecules. The resulting iPSC-derived ECs can be cultured for multiple passages without losing their functionality and are suitable for both in vitro and in vivo studies.