Paraneoplastic anti-neuronal nuclear IgG autoantibodies (type I) localize antigen in small cell lung carcinoma.
Paraneoplastic anti-neuronal nuclear IgG autoantibodies (type I) localize antigen in small cell lung carcinoma.
复制标题
副肿瘤性抗神经元核 IgG 自身抗体（I 型）定位小细胞肺癌中的抗原。
DOI：
10.1016/s0025-6196(12)62471-9
复制
发表时间：
1991
影响因子：
8.9
通讯作者：
Lennon,VA
中科院分区：
文献类型：
--
作者：
Kiers,L;Altermatt,HJ;Lennon,VA
I型抗神经元核自身抗体（ANNA-I，也称为"Hu"）是患有周围神经病或脑脊髓神经根病的患者中小细胞肺癌（SCLC）的独特血清学标志物。与这些抗体反应的肿瘤抗原已被其他研究者通过Western印迹分析鉴定，但抗原尚未在SCLC中定位。因此，我们测试，通过间接免疫荧光，49个连续ANNA-I阳性患者和30个对照组的血清IgG反应与小细胞肺癌。测试了两种肿瘤细胞系，一种是从患有Lambert-Eaton综合征的患者的原发性肿瘤建立的，第二种是从没有神经系统疾病的患者的转移性病变建立的。所有ANNA-I阳性血清中的IgG均与两种肿瘤结合。在大多数情况下，该模式类似于神经元中所见，具有强烈的均匀核染色，核仁稀疏，以及微弱的细胞质染色。在SCLC底物上获得的终点稀释度与在中枢和外周神经元上获得的终点稀释度之间注意到高度显著的相关性（r = 0.863; P <0.001）。30份对照血清中有3份IgG以低滴度与SCLC细胞结合，但不与神经元结合，9份对照血清含有非器官特异性抗核抗体（ANA）。抗核抗体IgG可被小细胞肺癌和结肠腺癌细胞的匀浆等量吸收。相反，ANNA-I IgG与小脑和肌间神经丛神经元的反应性仅被均质化的SCLC细胞吸收。这些发现表明，SCLC抗原占所有的神经元反应的ANNA-I。这种特异性的IgG可作为一种有用的试剂，用于鉴定手术病理和细胞学标本中的SCLC细胞。
Type I anti-neuronal nuclear autoantibodies (ANNA-I, also known as “Hu”) are a distinctive serologic marker of small cell lung carcinoma (SCLC) in patients who have peripheral neuropathies or encephalomyeloradiculopathies. A tumor antigen reactive with these antibodies has been identified by other investigators by Western blot analyses, but an antigen has not been localized in SCLC immunohistochemically. We therefore tested, by indirect immunofluorescence, the sera of 49 sequential ANNA-I-positive patients and 30 control subjects for IgG reactive with SCLC. Two tumor cell lines were tested, one established from the primary tumor of a patient with Lambert-Eaton syndrome and the second from the metastatic lesion of a patient without neurologic disease. IgG in all ANNA-I-positive sera bound to both tumors. In most instances, the pattern resembled that seen in neurons, with strong homogeneous nuclear staining, sparing of nucleoli, and faint cytoplasmic staining. A highly significant correlation was noted between endpoint dilutions obtained on SCLC substrates and on central and peripheral neurons (r= 0.863;P<0.001). IgG in 3 of 30 control sera bound in low titer to SCLC cells but not to neurons, and 9 control sera contained non-organ-specific anti-nuclear antibodies (ANA). The ANA IgG was absorbed equivalently by homogenates of SCLC or colonic adenocarcinoma cells. In contrast, the reactivity of ANNA-I IgG with cerebellar and myenteric plexus neurons was absorbed only by homogenized SCLC cells. These findings suggest that SCLC antigens account for all neuronal reactivity of ANNA-I. IgG of this specificity may serve as a useful reagent for identifying SCLC cells in surgical pathologic and cytologic specimens.