Mapping remodeling of thalamocortical projections in the living reeler mouse brain by diffusion tractography
Mapping remodeling of thalamocortical projections in the living reeler mouse brain by diffusion tractography
复制标题
DOI：
10.1073/pnas.1218330110
复制
发表时间：
2013-05-07
影响因子：
11.1
通讯作者：
Staiger, Jochen F.
中科院分区：
文献类型：
--
作者：
Harsan, Laura-Adela;David, Csaba;Staiger, Jochen F.
神经科学的一个主要挑战是在微观水平上准确地破译整个大脑回路（连接组）。目前，唯一能提供一个全局无创脑结构连接窗口的方法是弥漫性神经束造影。然而，重建的路径反映真实神经网络的程度取决于数据采集和后处理因素。通过独特的方法组合，我们设计并评估了一个可靠的纤维跟踪和绘制活体小鼠脑连接组的框架。一个重要的连接灰质区域和通过纤维交叉区域的线路方案被仔细检查：lemiscal thalamocor皮质（TC）通路。在相同的野生型和reeler突变小鼠中，我们定量验证了由体内神经束造影和相关组织学轴突示踪推断的TC投影。我们非侵入性地证明了皮层薄片模式的改变，如卷轴上高度无序的皮层层压，导致了TC通路的惊人代偿性重构。
A major challenge in neuroscience is to accurately decipher in vivo the entire brain circuitry (connectome) at a microscopic level. Currently, the only methodology providing a global noninvasive window into structural brain connectivity is diffusion tractography. The extent to which the reconstructed pathways reflect realistic neuronal networks depends, however, on data acquisition and postprocessing factors. Through a unique combination of approaches, we designed and evaluated herein a framework for reliable fiber tracking and mapping of the living mouse brain connectome. One important wiring scheme, connecting gray matter regions and passing fiber-crossing areas, was closely examined: the lemniscal thalamocortical (TC) pathway. We quantitatively validated the TC projections inferred from in vivo tractography with correlative histological axonal tracing in the same wild-type and reeler mutant mice. We demonstrated noninvasively that changes in patterning of the cortical sheet, such as highly disorganized cortical lamination in reeler, led to spectacular compensatory remodeling of the TC pathway.