Immunohistochemical studies on endothelial cell phenotype in hepatocellular carcinoma
Immunohistochemical studies on endothelial cell phenotype in hepatocellular carcinoma
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DOI：
10.1002/hep.510260222
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发表时间：
1997-08-01
期刊：
影响因子：
13.5
通讯作者：
Syed, AS
中科院分区：
文献类型：
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作者：
Nakamura, S;Muro, H;Syed, AS
为研究肝细胞癌（HCC）中肿瘤细胞周围窦状隙内皮细胞（SEC）的表型和毛细血管化过程，应用CD 4、CD 14单克隆抗体（MAbs）对51例原发性HCC、4例肾上腺转移瘤和3例门静脉癌栓进行了免疫组化染色（脂多糖结合蛋白复合物受体）和CD 32（Fc γ受体II），其特异性地存在于正常肝脏中的SEC上，但不在普通血管内皮细胞（EC）上。还对CD 36（血小板反应蛋白受体）、EN 4抗原（Ag）（泛血管内皮细胞抗原）、PALE抗原（静脉和毛细血管EC抗原）、VIII因子相关抗原（FVIIIRAg）和层粘连蛋白进行了免疫染色。识别巨噬细胞的MAb 25 F9与其他MAb同时用于区分HCC中的巨噬细胞和SEC（HCC SEC）。CD 4、CD 14和/或CD 32仅在12例分化良好的原发性HCC中在HCC SEC上发现，显示薄的小梁状或假腺状肿瘤细胞排列。这12例肿瘤均小于无CD 4、CD 14和/或CD 32阳性SECs的肿瘤（P
To examine the phenotype of the sinusoidal endothelial cells (SECs) surrounding tumor cells and the process of capillarization in hepatocellular carcinoma (HCC), 51 primary HCCs, 4 adrenal metastases, and 3 portal tumor thrombi were immunohistochemically stained with monoclonal antibodies (MAbs) for CD4, CD14 (lipopolysaccharide-binding protein complex receptors), and CD32 (Fc gamma receptor II), which are specifically found on the SECs in normal liver, but not on ordinary vascular endothelial cells (ECs). Immunostaining was also performed for CD36 (thrombospondin receptors), EN4 antigen (Ag) (a pan-vascular endothelial cell Ag), PALE Ag (a venous and capillary EC Ag), factor VIII-related Ag (FVIIIRAg), and laminin. MAb 25F9, which identifies macrophages, was simultaneously used with the other MAbs to distinguish macrophages from SECs in HCCs (HCC SECs). CD4, CD14, and/or CD32 were found on HCC SECs only in 12 well-differentiated primary HCCs showing a thin trabecular or pseudoglandular tumor cell arrangement. These 12 tumors were smaller than those without CD4-, CD14-, and/or CD32-positive SECs (P