Biodegradation of ethyl t-butyl ether (E TBE), methyl t-butyl ether (MTBE) and t-amyl methyl ether (TAME) by Gordonia terrae
Biodegradation of ethyl t-butyl ether (E TBE), methyl t-butyl ether (MTBE) and t-amyl methyl ether (TAME) by Gordonia terrae
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DOI：
10.1007/s002530000482
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发表时间：
2001-01-01
影响因子：
5
通讯作者：
Vandecasteele, JP
中科院分区：
文献类型：
--
作者：
Hernandez-Perez, G;Fayolle, F;Vandecasteele, JP
从活性污泥中筛选出一株能以乙基叔丁基醚(ETBE)为唯一碳源和能源生长的菌株IFP 2001。ETBE按化学计量比降解为叔丁醇(TBA)，活性是可诱导的。还选择了来源于菌株IFP 2001的本构菌株G.terrae IFP 2007。这两株菌株不以甲基叔丁基醚(MTBE)和叔戊基甲醚(TAME)为碳源，但在乙醇等碳源存在下，MTBE和TAME分别被共代谢为TEA和TAA。在ETBE上生长过程中没有检测到双碳化合物，但在MTBE或TAME的共代谢过程中产生了甲酸盐。ETBE在厌氧条件下没有降解，而在ETBE上培养诱导后的G.ten ae IFP 2001中存在细胞色素P-450，因此单加氧酶参与了醚类的降解。
Gordonia terrae strain IFP 2001 was selected from activated sludge for its capacity to grow on ethyl t-butyl ether (ETBE) as sole carbon and energy source. ETBE was stoichiometrically degraded to t-butyl alcohol (TBA) and the activity was inducible. A constitutive strain, G. terrae IFP 2007, derived from strain IFP 2001, was also selected. Methyl t-butyl ether (MTBE) and t-amyl methyl ether (TAME) were not used as carbon and energy sources by the two strains, but cometabolic degradation of MTBE and TAME was demonstrated, to TEA and t-amyl alcohol (TAA) respectively, in the presence of a carbon source such as ethanol. No two-carbon compound was detected during growth on ETBE, but formate was produced during cometabolic degradation of MTBE or TAME. A monooxygenase was involved in the degradation of ethers, because no degradation of ETBE was observed under anaerobic conditions and the presence of a cytochrome P-450 was demonstrated in G. ten ae IFP 2001 after induction by cultivation on ETBE.