Microarray analysis identifies genes preferentially expressed in the lung schistosomulum of Schistosoma mansoni
Microarray analysis identifies genes preferentially expressed in the lung schistosomulum of Schistosoma mansoni
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DOI：
10.1016/j.ijpara.2005.10.008
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发表时间：
2006-01-01
影响因子：
4
通讯作者：
Ivens, AC
中科院分区：
文献类型：
--
作者：
Dillon, GP;Feltwell, T;Ivens, AC
曼氏血吸虫的肺血吸虫是在接种疫苗的小鼠中激发保护性免疫的有效靶点。为了鉴定在这一阶段表达的基因，我们构建了一个微阵列，代表3088个重叠群和单链，来自体外培养的幼虫的cDNA，并用它来筛选七个生活史阶段的RNA。根据整个生命周期的表达谱对基因进行聚类，揭示了一些膜蛋白、膜相关蛋白和分泌蛋白在肺部阶段上调，这些蛋白可能代表潜在的免疫靶点。两个有希望的分泌分子与疫苗抗原和/或其他蠕虫的免疫调节潜力具有同源性。(C)2005澳大利亚寄生虫学会有限公司出版。保留所有权利。
The lung schistosomulum of Schistosoma mansoni is a validated target of protective immunity elicited in vaccinated mice. To identify genes expressed at this stage we constructed a microarray, representing 3088 contigs and singlets, with cDNA derived from in vitro cultured larvae and used it to screen RNA from seven life-cycle stages. Clustering of genes by expression profile across the life cycle revealed a number of membrane, membrane-associated and secreted proteins up-regulated at the lung stage, that may represent potential immune targets. Two promising secreted molecules have homology to antigens with vaccine and/or immunomodulatory potential in other helminths. (c) 2005 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.