Cloning and characterization of an albicidin resistance gene from Klebsiella oxytoca

Cloning and characterization of an albicidin resistance gene from Klebsiella oxytoca
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产酸克雷伯菌 Albicidin 抗性基因的克隆和表征

DOI:
10.1111/j.1365-2958.1988.tb00050.x
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发表时间:
1988
影响因子:
3.6
通讯作者:
J. M. Pemberton
J. M. Pemberton
中科院分区:
生物学2区
文献类型:
--
作者:
M. Walker;R. Birch;J. M. Pemberton

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从产酸克雷伯氏菌中分离出一个含有对抗生素albicidin具有抗性的基因的DNA片段,并显示其在大肠杆菌中表达,在大肠杆菌中它还保护噬菌体T7的复制不受albicidin的抑制。体内翻译分析表明,克隆的2.2kb DNA片段编码36 kD和25 kD蛋白。
A DNA fragment containing a gene for resistance to the antibiotic albicidin was isolated from Klebsiella oxytoca and shown to be expressed in Escherichia coli, where it also protected bacteriophage T7 replication from inhibition by albicidin. In vivo translation analysis demonstrated that the cloned 2.2kb DNA fragment coded for a 36 kiloDalton (kD) protein and a 25 kD protein.
DOI: 10.1073/pnas.82.4.1074
发表时间: 1985-01-01
影响因子: 11.1
作者:
TABOR, S;RICHARDSON, CC
通讯作者: RICHARDSON, CC