Immunocytochemistry of the pituitary glycoprotein hormones.
Immunocytochemistry of the pituitary glycoprotein hormones.
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垂体糖蛋白激素的免疫细胞化学。
DOI：
10.1177/24.7.60435
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发表时间：
1976
期刊：
影响因子：
--
通讯作者：
G. C. Moriarty
中科院分区：
文献类型：
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作者：
G. C. Moriarty
使用电子显微镜免疫细胞化学技术和促卵泡激素（FSH）、促黄体生成激素（LH）和促甲状腺激素（TSH）的β（β）链的抗血清鉴定垂体糖蛋白激素的储存位点。正常大鼠的TSH细胞呈卵圆形或角形，含有直径60-160 nm的小颗粒。在甲状腺切除后45天肥大的TSH细胞中，染色呈颗粒状的球状斑片或弥漫分布在扩张的粗面内质网的扩张轮廓中。促性腺激素细胞（FSH和LH细胞）表现出三种不同的形态。I型细胞为卵圆形，具有大颗粒群和小颗粒群。FSH β或LH β的染色仅在大颗粒（直径为400 nm或更大）中强烈且特异。II型细胞呈角状或星状，含有大量直径平均为200-220 nm的分泌颗粒。它们在发情周期中FSH或LH分泌高的阶段占优势。III型细胞看起来像促肾上腺皮质激素（ACTH）细胞，因为它们是星状的，周围排列着颗粒。它们通常仅用抗FSH β染色，并且它们的染色不能通过加入100 ng ACTH而消除。在初步的定量研究骑自行车的女性，我们发现，在连续切片FSH细胞和LH细胞表现出类似的变化，以更角的细胞群体在活跃的分泌阶段。然而，这些转变彼此并不同步。此外，在周期的所有阶段，FSH细胞至少是LH细胞的1.5倍。我们收集的系列细胞显示，一些细胞（通常是I型或II型）对两种促性腺激素都染色，而另一些细胞（通常是II型或III型）只含有一种促性腺激素。
The storage sites of the pituitary glycoprotein hormones were identified with the use of electron microscopic immunocytochemical techniques and antisera to the beta (beta) chains of follicle-stimulating hormone (FSH), luteinizing hormone (LH) and thyroid-stimulating hormone (TSH). The TSH cells in normal rats is ovoid or angular and contains small granules 60-160 nm in diameter. In TSH cells hypertrophied 45 days after thyroidectomy, staining is in globular patches in granules or diffusely distributed in the expanded profiles of dilated rough endoplasmic reticulum. The gonadotrophs (FSH and LH cells) exhibited three different morphologies. Type I cells are ovoid with a population of large granules and a population of small granules. Staining for FSHbeta or LHbeta was intense and specific only in the large granules (diameter of 400 nm or greater). Type II cells are angular or stellate and contain numerous secretory granules averaging 200-220 nm in diameter. They predominate during stages in the estrous cycle when FSH or LH secretion is high. Type III cells look like adrenocorticotropin (ACTH) cells in that they are stellate with peripherally arranged granules. They generally stain only with anti-FSHbeta and their staining can not be abolished by the addition of 100 ng ACTH. In preliminary quantitative studies of cycling females, we found that on serial sections FSH cells and LH cells show similar shifts to a more angular population of cells during stages of active secretion. However, the shifts are not in phase with one another. Furthermore, there are at least 1.5 times more FSH cells than LH cells at all stages of the cycle. Our collection of serial cells shows that some cells (usually type I or II) stain for both gonadotropic hormones, whereas others (usually type II or III) contain only one.