Expression of platelet-derived growth factor-A (PDGF-A), PDGF-B, and PDGF receptor-alpha and -beta during human testicular development and disease.
Expression of platelet-derived growth factor-A (PDGF-A), PDGF-B, and PDGF receptor-alpha and -beta during human testicular development and disease.
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发表时间：
2002
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通讯作者：
S. Basciani;S. Mariani;M. Arizzi;S. Ulisse;N. Rucci;E. Jannini;C. Della Rocca;A. Manicone;C. Carani;G. Spera;L. Gnessi
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作者：
S. Basciani;S. Mariani;M. Arizzi;S. Ulisse;N. Rucci;E. Jannini;C. Della Rocca;A. Manicone;C. Carani;G. Spera;L. Gnessi
血小板衍生生长因子(PDGF)在调节多种组织的发育和功能调控中起着关键作用，并与癌症和动脉粥样硬化等严重疾病的发病机制有关。鉴于PDGF在男性性腺发育和功能中的新作用，我们比较了胎儿和成人睾丸中PDGF A链和B链以及PDGF受体(PDGFR)α和β亚基的mRNAs的表达谱。并对胎儿、成人和病变人睾丸组织中相应蛋白的免疫组织化学定位进行了分析。Northern分析和RT-PCR均可检测到PDGFs和PDGFRs的mRNAs。在妊娠16-20周，转录水平较高，在24-28周显著降低，在成年后显著升高。免疫组织化学证实了相同的蛋白表达模式，尽管PDGF系统的细胞定位在出生后发育过程中发生变化，伴随着精子发生的进展。在生殖细胞完全性发育不全或不同程度生精障碍患者的睾丸组织中，PDGFs及其受体的免疫组织化学定位与正常不同，证实生殖细胞与PDGF系统的分布密切相关。这些结果表明，PDGF可能通过复杂的相互作用在人类的个体发育和睾丸病理生理中发挥主导作用。最后，PDGF配体和受体蛋白在间质细胞肿瘤中的表达提示PDGF系统与该睾丸肿瘤的肿瘤发生或肿瘤进展有关。
Platelet-derived growth factor (PDGF) plays a critical role in regulating the development and functional control of various tissues and has been implicated in the pathogenesis of serious diseases, including cancer and atherosclerosis. Given the emerging role of PDGF in the development and function of male gonads, we compared the expression profiles of the mRNAs of the PDGF A- and B-chains and of the PDGF receptor (PDGFR) alpha- and beta-subunits in fetal and adult human testis. The immunohistochemical localization of the corresponding proteins in fetal, adult, and diseased human testicular tissues was also analyzed. PDGFs and PDGFRs mRNAs were readily detected by both Northern analysis and RT-PCR. The transcript levels were higher during 16-20 wk gestation, significantly lower at 24-28 wk, and increased in the adult. An identical pattern of protein expression was confirmed by immunohistochemistry, although the cellular localization of the PDGF system changes during postnatal development, concomitantly with the progression of spermatogenesis. In the testicular samples from patients affected by either complete aplasia of germ cells or various grades of spermatogenic arrest, the immunohistochemical localization of PDGFs and PDGFRs was different from normal, confirming a close connection between germ cells and PDGF system distribution. These results indicate that PDGF, through complex interactions, could play a leading role in ontogenesis and testicular pathophysiology in humans. Finally, the expression of PDGF ligands and receptor proteins in Leydig cell tumors suggests a relationship of the PDGF system to tumorigenesis or tumor progression in this testicular neoplasm.