Reduction of glutamine synthetase specific activity in cultured astroglia by ferrous chloride.
Reduction of glutamine synthetase specific activity in cultured astroglia by ferrous chloride.
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氯化亚铁降低培养的星形胶质细胞中谷氨酰胺合成酶的比活性。
DOI：
10.1002/jnr.490240408
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发表时间：
1989
影响因子：
4.2
通讯作者：
Sheeler-Gough,LV
中科院分区：
文献类型：
--
作者：
Tiffany-Castiglioni,E;Roberts,JA;Sheeler-Gough,LV
培养中的未成熟和成熟大鼠星形胶质细胞在单次暴露于致痫原 FeCl2 后测定谷氨酰胺合成酶 (GS) 活性。使用标准浓度和升高的细胞外钾或谷氨酸 (Glu) 浓度培养细胞。 FeCl2 将 GS 活性降低至对照水平以下，而高 Glu 则增加 GS 活性。然而，在给予 FeCl2 和高 Glu 的培养物中，高 Glu 的刺激显着减弱，这表明用 FeCl2 处理的细胞不能通过增加其 GS 活性来有效地响应增加的细胞外谷氨酸。这些发现的意义在于，基于 GS 在大脑氨解毒中的重要性，神经胶质细胞对神经元环境的调节可能会受到损害。
Immature and mature rat astroglia in culture were assayed for glutamine synthetase (GS) activity after a single exposure to the epileptogen FeCl2. Cells were cultured with both standard and elevated extracellular potassium or glutamate (Glu) concentration. FeCl2reduced GS activity below control levels, whereas high Glu increased GS activity. However, stimulation by high Glu was significantly attenuated in cultures given both FeCl2and high Glu, indicating that cells treated with FeCl2were not able to respond as effectively to increased extracellular glutamate by increasing their GS activity. The significance of these findings is that glial regulation of the neuronal environment may be impaired, based on the proposed importance of GS in ammonia detoxification in the brain.