FGF induces new feather buds from developing avian skin.
FGF induces new feather buds from developing avian skin.
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FGF 诱导鸟类皮肤发育出新的羽芽。
DOI：
10.1111/1523-1747.ep12330553
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Chuong,CM
中科院分区：
文献类型：
--
作者：
Widelitz,RB;Jiang,TX;Noveen,A;Chen,CW;Chuong,CM
皮肤附属物的诱导涉及一系列分子事件。成纤维细胞生长因子(成纤维细胞生长因子)家族多肽生长因子参与细胞增殖和形态发生。我们以发育中的鸡羽芽为模型，探讨了FGFs在皮肤附属物诱导过程中的作用。将成纤维细胞生长因子-1、成纤维细胞生长因子-2或成纤维细胞生长因子-4直接添加到培养基中或从预先浸泡的阿菲格尔蓝珠中释放出来。在中线附近，FGFs导致发育中的羽芽融合，代表FGFs在发育中的皮肤中扩展羽芽域的能力。在未形成羽状胎座的外植体的外侧区域，用成纤维细胞生长因子处理产生了一个凝聚区和一个羽芽数量增加的区域。在通常无羽毛的腹侧表皮中，FGFs也能诱导出新的羽状芽。与正常羽毛芽一样，新诱导的羽芽表达Shh。GRB、Ras、Raf和Erk是已知位于酪氨酸激酶受体下游的细胞内信号分子，如成纤维细胞生长因子受体，在羽芽结构域中表达丰富。酪氨酸激酶抑制剂金雀异黄素可抑制羽芽的形成和成纤维细胞生长因子的作用。这些结果表明，不同的上皮功能对FGFs有不同的反应。然而，所有的表型反应表明，FGFs促进了皮肤附着域的形成。
Induction of skin appendages involves a cascade of molecular events. The fibroblast growth factor (FGF) family of peptide growth factors is involved in cell proliferation and morphogenesis. We explored the role of the FGFs during skin appendage induction using developing chicken feather buds as a model. FGF-1, FGF-2, or FGF-4 was added directly to the culture medium or was released from pre-soaked Affigel blue beads. Near the midline, FGFs led to fusion of developing feather buds, representing FGFs' ability to expand feather bud domains in developing skin. In lateral regions of the explant where feather placodes have not formed, FGF treatment produces a zone of condensation and a region with an increased number of feather buds. In ventral epidermis that is normally apteric (without feathers), FGFs can also induce new feather buds. Like normal feather buds, the newly induced buds express Shh. The expression of Grb, Ras, Raf, and Erk, intracellular signaling molecules known to be downstream to tyrosine kinase receptors such as the FGF receptor, was enriched in feather bud domains. Genistein, an inhibitor of tyrosine kinase, suppressed feather bud formation and the effect of FGF. These results indicate that there are varied responses to FGFs depending on epithelial competence. All the phenotypic responses, however, show that FGFs facilitate the formation of skin appendage domains.