Inheritance of chromosomally integrated human herpesvirus 6 DNA.
Inheritance of chromosomally integrated human herpesvirus 6 DNA.
复制标题
染色体整合的人类疱疹病毒 6 DNA 的遗传。
DOI：
10.1182/blood.v94.5.1545
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发表时间：
1999
期刊：
影响因子：
20.3
通讯作者：
Isao Miyoshi
中科院分区：
文献类型：
--
作者：
M. Daibata;Takahiro Taguchi;Y. Nemoto;Hirokuni Taguchi;Isao Miyoshi
人类疱疹病毒6 （HHV-6）基因组已在几种没有活动性病毒感染迹象的人类淋巴增生性疾病中被检测到，并在某些情况下被发现整合到染色体中。我们之前报道了一名妇女感染hhv -6伯基特淋巴瘤。荧光原位杂交显示病毒基因组被整合到22号染色体（22q13）的长臂上。患者无症状的丈夫也携带整合在染色体位点1q44的HHV-6 DNA。为了评估HHV-6 DNA染色体传播的可能性，我们在他们女儿的外周血中寻找HHV-6 DNA。她在22q13和1q44两条染色体上都有HHV-6 DNA，分别与她母亲和父亲的病毒整合位点相同。研究结果表明，她的病毒基因组在染色体上遗传自父母双方。3名家庭成员HHV-6血清学检测均呈阳性，但无活动性感染的血清学征象。为了证实HHV-6 DNA序列的存在，我们对针对HHV-6不同区域的7对不同引物进行了聚合酶链反应（PCR）。单步PCR检测到的病毒序列在所有3个家族成员中一致。我们提出了一种新的HHV-6潜伏形式，其中整合的病毒基因组可以染色体传播。染色体整合的HHV-6在淋巴增生性疾病发病机制中的可能作用仍有待解释。
Human herpesvirus 6 (HHV-6) genome has been detected in several human lymphoproliferative disorders with no signs of active viral infection, and found to be integrated into chromosomes in some cases. We previously reported a woman with HHV-6-infected Burkitt's lymphoma. Fluorescence in situ hybridization showed that the viral genome was integrated into the long arm of chromosome 22 (22q13). The patient's asymptomatic husband also carried HHV-6 DNA integrated at chromosome locus 1q44. To assess the possibility of chromosomal transmission of HHV-6 DNA, we looked for HHV-6 DNA in the peripheral blood of their daughter. She had HHV-6 DNA on both chromosomes 22q13 and 1q44, identical to the site of viral integration of her mother and father, respectively. The findings suggested that her viral genomes were inherited chromosomally from both parents. The 3 family members were all seropositive for HHV-6, but showed no serological signs of active infection. To confirm the presence of HHV-6 DNA sequences, we performed polymerase chain reaction (PCR) with 7 distinct primer pairs that target different regions of HHV-6. The viral sequences were consistently detected by single-step PCR in all 3 family members. We propose a novel latent form for HHV-6, in which integrated viral genome can be chromosomally transmitted. The possible role of the chromosomally integrated HHV-6 in the pathogenesis of lymphoproliferative diseases remains to be explained.
3例患者B细胞淋巴瘤组织中人B淋巴细胞病毒（人疱疹病毒6）序列的检测。
影响因子：
11.4
作者：
Josephs,SF;Buchbinder,A;Streicher,HZ;Ablashi,DV;Salahuddin,SZ;Guo,HG;Wong-Staal,F;Cossman,J;Raffeld,M;Sundeen,J
通讯作者：
Sundeen,J